Immunofluorescent analysis of Cytochrome C (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a Cytochrome C mouse monoclonal antibody (Product # 33-8200) at a dilution of 5ug/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst Dye (Product # 62249). Images were taken at 20X magnification on a Thermo Scientific ToxInsight HCS imaging instrument.
|Tested species reactivity||Fruit fly, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Human, Chicken|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Rat Cytochrome C.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1 µg/ml|
|Immunofluorescence (IF)||5-20 µg/ml|
|Immunoprecipitation (IP)||15 µg|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.
IP-MS enrichment of CYCS (LFQ intensity): CYCS was enriched 489-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and CYCS antibody (Part No. 33-8200). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Parkinson's disease transgenic mitochondrial cybrids generate Lewy inclusion bodies.
33-8200 was used in immunocytochemistry to report the generation of fibrillar and vesicular inclusions in a long-term cybrid cell culture model used to study Parkinson's disease.
|Trimmer PA,Borland MK,Keeney PM,Bennett JP,Parker WD||Journal of neurochemistry (88:800)||2004|
Afferent regulation of cytochrome-c and active caspase-9 in the avian cochlear nucleus.
33-8200 was used in immunohistochemistry (free floating) to study apoptosis of nucleus magnocellularis neurons.
|Wilkinson BL,Elam JS,Fadool DA,Hyson RL||Neuroscience (120:1071)||2003|
|Human||Not Cited||Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.||Love DC,Kochan J,Cathey RL,Shin SH,Hanover JA,Kochran J||Journal of cell science (116:647)||2003|
|Human||1:600||The Toxoplasma gondii protein ROP2 mediates host organelle association with the parasitophorous vacuole membrane.||Sinai AP,Joiner KA||The Journal of cell biology (154:95)||2001|
|Human||Not Cited||Elevated AKT activity protects the prostate cancer cell line LNCaP from TRAIL-induced apoptosis.||Nesterov A,Lu X,Johnson M,Miller GJ,Ivashchenko Y,Kraft AS||The Journal of biological chemistry (276:10767)||2001|