|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified bovine cytochrome c protein|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-9586 detects Cytochrome C in human, mouse, bovine and rat samples.
PA1-9586 has been successfully used in Western blot and ELISA procedures. By Western blot, PA1-9586 detects a 12 kDa protein representing Cytochrome C. Do not use dry milk in blocking or dilution buffer in your assay. Use BSA (3-5% in blocking and 1-3% in dilution buffers) in your assay.
PA1-9586 immunogen is a purified bovine cytochrome c protein
Store at 4°C for 1 week, and -20°C for up to one year.
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Mineralocorticoid receptor mediated liposomal delivery system for targeted induction of apoptosis in cancer cells.
PA1-9586 was used in western blot to characterize the mineralocorticoid receptor mediated liposomal delivery system to induce apoptosis in cancer cells
|Sharma P,Banerjee R,Narayan KP||Biochimica et biophysica acta (1858:415)||2016|