Western blot analysis of cytochrome C was performed by loading 20 µg of MCF-7 (lane 1) and MDA-MB-231 (lane 2) cell lysates in RIPA buffer (Product # 89901) and Page Ruler Plus Protein Ladder (Product # 26620) onto a 4-8% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE). Proteins were transferred to nitrocellulose membrane blocked in 5% milk /PBST for one hour at room temperature. Cytochrome C was detected using a cytochrome C monoclonal antibody (Product # 45-6100) at a dilution of 1:500 5% milk /PBST overnight at at 4°C on a rocking platform, followed by a Goat anti-Mouse IgG (H + L) -HRP secondary antibody at a dilution of 1:5,000 for one hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34078) and Kodak M35 X-OMAT Automatic Processor. Data courtesy of Dr. Wei Xu at the University of Wisconsin, Madison, WI.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||0.5-2 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
45-6100 was used successfully in the western blot analysis of cytochrome C using human breast cancer cell line lysates.
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain.
45-6100 was used in immunohistochemistry - paraffin section to test if the severe neurodegeneration observed in mutUNG1-expressing mice is prevented by a ketogenic diet
|Lauritzen KH,Hasan-Olive MM,Regnell CE,Kleppa L,Scheibye-Knudsen M,Gjedde A,Klungland A,Bohr VA,Storm-Mathisen J,Bergersen LH||Neurobiology of aging (48:34)||2016|
Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.
45-6100 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.
|Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A||DNA repair (10:639)||2011|
Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.
45-6100 was used in western blot to investigate the impact of Tmem65 on heart development
|Sharma P,Abbasi C,Lazic S,Teng AC,Wang D,Dubois N,Ignatchenko V,Wong V,Liu J,Araki T,Tiburcy M,Ackerley C,Zimmermann WH,Hamilton R,Sun Y,Liu PP,Keller G,Stagljar I,Scott IC,Kislinger T,Gramolini AO||Nature communications (6:null)||2015|
Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons.
45-6100 was used in western blot to investigate the effect of p53 acetylation on DNA damage-induced neuronal apoptosis.
|Brochier C,Dennis G,Rivieccio MA,McLaughlin K,Coppola G,Ratan RR,Langley B||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:8621)||2013|
|Not Applicable||Not Cited||
Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1.
45-6100 was used in western blot to report the 19-A 3D map of the 1.7-MDa amphipol-solubilized supercomplex I(1)III(2)IV(1) isolated from bovine heart
|Althoff T,Mills DJ,Popot JL,Kühlbrandt W||The EMBO journal (30:4652)||2011|