Invitrogen

Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor™ 647

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Cytochrome C Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Cytochrome C (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cytochrome C monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-38200-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Product Details

MA3-38200-A647

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	1-10 µg/mL	-

Product Specifications
Species Reactivity	Fruit fly, Human, Mouse, Rabbit, Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	6H2.B4
Immunogen	Rat cytochrome C
Conjugate	Alexa Fluor™ 647 Alexa Fluor 488 Alexa Fluor 555 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2663052

Product Specific Information

This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CG17903-PA; CG17903-PB; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p

Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4

UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029

Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996, (Rabbit) 100341175, (Rabbit) 100357840

Function(s)