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Invitrogen
Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor™ 647
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FIGURE: 1 / 1
Cytochrome C Antibody (MA3-38200-A647) in ICC/IF
Immunofluorescent analysis of Cytochrome C (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cytochrome C monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-38200-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at ... View More
Product Details
MA3-38200-A647
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6H2.B4
Immunogen
Rat cytochrome C
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2663052
Product Specific Information
This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG17903-PA; CG17903-PB; CRF; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p
Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4
UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029
Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996, (Rabbit) 100341175, (Rabbit) 100357840
response to reactive oxygen species
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
protein dephosphorylation
mitochondrion organization
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
cellular respiration
intrinsic apoptotic signaling pathway
apoptotic DNA fragmentation
apoptotic process
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
oxidative phosphorylation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
oxidation-reduction process
response to ischemia
response to oxidative stress
negative regulation of hydrogen peroxide biosynthetic process
glial cell apoptotic process
positive regulation of cellular respiration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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