Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c.
Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis, and immunoprecipitation. (Fluorochrome conjugated 6H2 is recommended for use in intracellular flow cytometry.).
Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1
Gene Aliases: CYC; CYCS; CYCSA; HCS; THC4