|Tested species reactivity||Fruit fly, Human, Mouse, Rabbit, Rat, Xenopus|
|Published species reactivity||Rat, Fish, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Equine Cytochrome c.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tumor protein Tctp regulates axon development in the embryonic visual system.
33-8500 was used in western blot to assess regulation of axon development in the embryonic visual system by tumor protein Tctp
|Roque CG,Wong HH,Lin JQ,Holt CE||Development (Cambridge, England) (143:1134)||2016|
Morphine stimulates nitric oxide release in human mitochondria.
33-8500 was used in western blot to discuss morphinergic/NO-coupled regulation.
|Stefano GB,Mantione KJ,Capellan L,Casares FM,Challenger S,Ramin R,Samuel JM,Snyder C,Kream RM||Journal of bioenergetics and biomembranes (47:409)||2015|
Lindane and cell death: at the crossroads between apoptosis, necrosis and autophagy.
33-8500 was used in western blot to examine the effects of lindane on autophagic, apoptotic, and necrotic cell death in rat hepatocytes
|Zucchini-Pascal N,de Sousa G,Rahmani R||Toxicology (256:32)||2009|
|Fish||Not Cited||Cadmium-induced apoptosis through the mitochondrial pathway in rainbow trout hepatocytes: involvement of oxidative stress.||Risso-de Faverney C,Orsini N,de Sousa G,Rahmani R||Aquatic toxicology (Amsterdam, Netherlands) (69:247)||2004|
|Human||Not Cited||Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis.||Reiss U,Oskouian B,Zhou J,Gupta V,Sooriyakumaran P,Kelly S,Wang E,Merrill AH,Saba JD||The Journal of biological chemistry (279:1281)||2004|
|Galectin-3 translocates to the perinuclear membranes and inhibits cytochrome c release from the mitochondria. A role for synexin in galectin-3 translocation.||Yu F,Finley RL,Raz A,Kim HR||The Journal of biological chemistry (277:15819)||2002|
|Not Applicable||Not Cited||
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism.
33-8500 was used in western blot to elucidate the mechanisms that regulate cycloheximide-induced apoptosis
|Tang D,Lahti JM,Grenet J,Kidd VJ||The Journal of biological chemistry (274:7245)||1999|
The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release.
33-8500 was used in immunoprecipitation to study the interaction between BRCA1 tumor suppressor and inositol 1,4,5-trisphosphate receptors and its effect on apoptotic calcium release.
|Hedgepeth SC,Garcia MI,Wagner LE,Rodriguez AM,Chintapalli SV,Snyder RR,Hankins GD,Henderson BR,Brodie KM,Yule DI,van Rossum DB,Boehning D||The Journal of biological chemistry (290:7304)||2015|
|Human||Not Cited||Targeting the GD3 acetylation pathway selectively induces apoptosis in glioblastoma.||Birks SM,Danquah JO,King L,Vlasak R,Gorecki DC,Pilkington GJ||Neuro-oncology (13:950)||2011|
|Human||Not Cited||Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase.||Love DC,Kochan J,Cathey RL,Shin SH,Hanover JA,Kochran J||Journal of cell science (116:647)||2003|
Protective effect of sulforaphane against cisplatin-induced mitochondrial alterations and impairment in the activity of NAD(P)H: quinone oxidoreductase 1 and ¿ glutamyl cysteine ligase: studies in mitochondria isolated from rat kidney and in LLC-PK1 cells.
33-8500 was used in western blot to test if sulforaphane alters cisplatin-induced mitochondrial alterations and impairs cytoprotective enzyme activity
|Guerrero-Beltrán CE,Calderón-Oliver M,Martínez-Abundis E,Tapia E,Zarco-Márquez G,Zazueta C,Pedraza-Chaverri J||Toxicology letters (199:80)||2010|
Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation.
33-8500 was used in western blot to report that PARP-1 contributes to mitochondrial impairment that ultimately leads to neuronal apoptosis after cerebral ischemia.
|Tanaka S,Takehashi M,Iida S,Kitajima T,Kamanaka Y,Stedeford T,Banasik M,Ueda K||Journal of neurochemistry (95:179)||2005|
Mycobacterium tuberculosis virulence correlates with mitochondrial cytochrome c release in infected macrophages.
33-8500 was used in western blot to examine mitochondrial function in the course of intracellular infections.
|Abarca-Rojano E,Rosas-Medina P,Zamudio-Cortéz P,Mondragón-Flores R,Sánchez-García FJ||Scandinavian journal of immunology (58:419)||2003|
The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space.
33-8500 was used in western blot to report that OPA1 resides in the mitochondria.
|Olichon A,Emorine LJ,Descoins E,Pelloquin L,Brichese L,Gas N,Guillou E,Delettre C,Valette A,Hamel CP,Ducommun B,Lenaers G,Belenguer P||FEBS letters (523:171)||2002|
Mitochondrial disruption in Drosophila apoptosis.
33-8500 was used in immunohistochemistry and western blot to study how Reaper, Hid, and Drp1 regulate mitochondrial disruption in fly apoptosis
|Abdelwahid E,Yokokura T,Krieser RJ,Balasundaram S,Fowle WH,White K||Developmental cell (12:793)||2007|