Invitrogen

Cytochrome C Recombinant Rabbit Monoclonal Antibody (ARC1153)

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Cytochrome C Antibody in Western Blot (WB) — Western Blot analysis of Cytochrome C performed on various cell lines using a Cytochrome C monoclonal antibody (Product # MA5-35607) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1153

Immunogen

A synthesized peptide derived from human Cytochrome C

Conjugate

Unconjugated

Form

Liquid

Concentration

0.405 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2849507

Target Information

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1

Gene Aliases: CYC; CYCS; CYCSA; HCS; THC4

UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898

Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309

response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic DNA fragmentation apoptotic process hydrogen peroxide metabolic process ATP synthesis coupled electron transport response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration

Process(es)

protein serine/threonine phosphatase activity protein binding heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding electron carrier activity iron ion binding lipid binding