|Tested species reactivity||Human|
|Host / Isotype||Mouse / Ig Mix|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||3.6 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ApoTrack™ Cytochrome c WB Cocktail (Sku# 459160) provides a means to detect apoptosis while detecting release of cytochrome c, as well as a cytoplasmic and mitochondrial markers with the help of western blot. This cocktail provides a means to detect cytochrome c, as well as a cytoplasmic and mitochondrial markers
Antibodies Cocktail Contains:
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse anti GAPDH monclonal:
Working concentration: 0.1 µg/ml
Mouse anti PDH-E1-alpha monoclonal:
Working concentration: 2 µg/ml
Mouse anti C-V-alpha monoclonal:
Working concentration: 0.5 µg/ml
This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.