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NeoBiotechnologies
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1-2 µg/mL | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1-2 µg/mL | - |
Flow Cytometry (Flow) |
1-2 µg/10^6 cells | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Amphibian, Avian, Dog, Fruit fly, Horse, Human, Mouse, Rat |
Host/Isotype |
Mouse / IgG1, kappa |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Clone |
rCYCS, 1010 |
Immunogen |
Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS, pH 7.4 |
Contains |
no preservative |
Storage conditions |
-20°C or -80°C if preferred |
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CG17903-PA; CG17903-PB; Cyt-c-p-PA; Cyt-c-p-PB; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-2; cytochrome c-like protein; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; hc1; lethal (2) k13905; RH17228p
Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; HCS; l(2)k13905; pDMc01; THC4
UniProt ID: (Dog) P00011, (Human) P99999, (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029
Entrez Gene ID: (Dog) 475258, (Human) 54205, (Horse) 100053958, (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996
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