Immunofluorescence analysis of Cytochrome P450 1A1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome P450 1A1 Rabbit Polyclonal Antibody (PA1340) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified, His-tagged, full length human P450 1A1 fusion protein.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PA1-340 detects cytochrome P450 (P450) 1A1 from human cells. This antibody detects, to a lesser extent, other P450 isoforms.
PA1-340 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~54 kDa protein representing P450 1A1 from MCF-7 cell extract.
The PA1-340 immunogen is purified, His-tagged, full length human P450 1A1 fusion protein.
The cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Detoxification: a novel function of BRCA1 in tumor suppression?
PA1-340 was used in western blot to investigate the involvement of BRCA1 in the prevention and repair of DNA damage
|Kang HJ,Hong YB,Kim HJ,Rodriguez OC,Nath RG,Tilli EM,Albanese C,Chung FL,Kwon SH,Bae I||Toxicological sciences : an official journal of the Society of Toxicology (122:26)||2011|
A novel in vitro pancreatic carcinogenesis model.
PA1-340 was used in western blot to evaluate an in vitro model of pancreatic carcinogenesis
|Kang HJ,Hong YB,Kim HJ,Yi YW,Nath RG,Chang YS,Cho HC,Bae I||Toxicology letters (202:15)||2011|
Identification through microarray gene expression analysis of cellular responses to benzo(a)pyrene and its diol-epoxide that are dependent or independent of p53.
PA1-340 was used in western blot to study p53-dependent and p53-independent responses to benzo(a)pyrene and its diol epoxide using DNA microarray analysis of gene expression
|Hockley SL,Arlt VM,Jahnke G,Hartwig A,Giddings I,Phillips DH||Carcinogenesis (29:202)||2008|