Immunohistochemistry analysis of Cytochrome P450 3A7 showing positive staining in the cytoplasm of paraffin-treated Human prostate carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytochrome P450 3A7 monoclonal antibody (Product # MA3-034) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Bovine, Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Clone||F19 P2 H2|
|Immunogen||Synthetic peptide corresponding to residues E S R D E T V S G A, conjugated to ovalbumin.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||2µg per 10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA3-034 detects Cytochrome P450 3A7 from Human samples and bovine liver samples.
MA3-034 has been successfully used in Western blot and Immunohistochemistry (paraffin) applications. By Western blot, this antibody detects a ~54 kDa band representing Cytochrome P450 3A7.
The MA3-034 immunogen is a synthetic peptide corresponding to residues E S R D E T V S G A, conjugated to ovalbumin.
This gene, CYP3A7, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. The enzyme also metabolizes some drugs such as aflatoxin B1. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Transcript variants have been described, but it is not known whether these transcripts are normally produced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cytochrome p450 profile of colorectal cancer: identification of markers of prognosis.
MA3-034 was used in immunohistochemistry to investigate the changes of cytochrome p450 expression in colorectal tumors
|Kumarakulasingham M,Rooney PH,Dundas SR,Telfer C,Melvin WT,Curran S,Murray GI||Clinical cancer research : an official journal of the American Association for Cancer Research (11:3758)||2005|