Immunofluorescent analysis of Cytochrome P450 4A (green) showing staining in the cytoplasm and membrane of H-4-II-E cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 4A polyclonal antibody (Product # PA3-033) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Cat, Human, Rabbit, Rat|
|Published species reactivity||Rat, Pig, Hamster, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-033 detects cytochrome P450 4A (isoforms 1, 2, and 3) from human, feline, rabbit and rat tissues.
PA3-033 has been successfully used in Western blot, ICC/IF and IHC procedures. By Western blot, this antibody detects an ~50 kDa protein representing P450 4A in rat liver extract. Immunohistochemical staining of P450 4A in rat brain with PA3-033 results in perivascular and surrounding cortex staining.
The PA3-033 immunogen is a synthetic peptide corresponding to residues D(431) P S R F A P D S P R H S H S(445) of rat Cytochrome P450 4A.
The cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA and quote;s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and w-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). 20-HETE is converted from AA by the 4A family of P450 proteins which includes at least 8 different, though closely related isoforms. 4A1, 4A2, and 4A3 have been cloned from liver, kidney and testis and have been detected in renal, hepatic and brain microvessels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression of CYP 4A ¿-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes.
PA3-033 was used in immunocytochemistry and western blot to utilize cultured rat brain astrocytes to study the expression of CYP 4A omega-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE)
|Gebremedhin D,Zhang DX,Carver KA,Rau N,Rarick KR,Roman RJ,Harder DR||Prostaglandins and other lipid mediators (124:16)||2016|
Beneficial effects of ß-conglycinin on renal function and nephrin expression in early streptozotocin-induced diabetic nephropathy rats.
PA3-033 was used in western blot to study the effects of beta-conglycin and soya isoflavone compounds in a rat model of diabetic nephropathy
|Yang HY,Wu LY,Yeh WJ,Chen JR||The British journal of nutrition (111:78)||2014|
Effects of soy protein on alcoholic liver disease in rats undergoing ethanol withdrawal.
PA3-033 was used in western blot to investigate the effectiveness of soy protein in decreasing the level of ethanol on alcoholic liver disease
|Yang HY,Lin HS,Chao JC,Chien YW,Peng HC,Chen JR||The Journal of nutritional biochemistry (23:679)||2012|
Modulation by salt intake of the vascular response mediated through adenosine A(2A) receptor: role of CYP epoxygenase and soluble epoxide hydrolase.
PA3-033 was used in western blot to investigate the role of adenosine A(2A) receptor in the vascular response modulated by high salt intake
|Nayeem MA,Zeldin DC,Boegehold MA,Morisseau C,Marowsky A,Ponnoth DS,Roush KP,Falck JR||American journal of physiology. Regulatory, integrative and comparative physiology (299:R325)||2010|
7-Benzyloxyresorufin-O-dealkylase activity as a marker for measuring cytochrome P450 CYP3A induction in mouse liver.
PA3-033 was used in western blot to investigate the relationship between 7-Benzyloxyresorufin-O-dealkylase activity and cytochrome P450 CYP3A induction in mouse liver
|Hagemeyer CE,Bürck C,Schwab R,Knoth R,Meyer RP||Analytical biochemistry (398:104)||2010|
Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6.
PA3-033 was used in western blot to investigate the role of ADP-ribosylation factor 6 (Arf6) in peroxisome biogenesis
|Anthonio EA,Brees C,Baumgart-Vogt E,Hongu T,Huybrechts SJ,Van Dijck P,Mannaerts GP,Kanaho Y,Van Veldhoven PP,Fransen M||BMC cell biology (10:null)||2009|
High dietary salt reduces the contribution of 20-HETE to arteriolar oxygen responsiveness in skeletal muscle.
PA3-033 was used in western blot to investigate the effects of high dietary salt on arteriolar oxygen responsiveness in skeletal muscle
|Marvar PJ,Falck JR,Boegehold MA||American journal of physiology. Heart and circulatory physiology (292:H1507)||2007|
Altered renal P-450 metabolism of arachidonic acid in Dahl salt-sensitive rats.
PA3-033 was used in western blot to investigate the metabolism of arachidonic acid by P-450 in Dahl salt-sensitive and salt-resistant rats
|Ma YH,Schwartzman ML,Roman RJ||The American journal of physiology (267:R579)||1994|
Attenuation of neonatal ischemic brain damage using a 20-HETE synthesis inhibitor.
PA3-033 was used in immunohistochemistry to investigate the effect of 20-hydroxyeicosatetraenoic acid synthesis inhibitor HET0016 on neonatal ischemic brain damage
|Yang ZJ,Carter EL,Kibler KK,Kwansa H,Crafa DA,Martin LJ,Roman RJ,Harder DR,Koehler RC||Journal of neurochemistry (121:168)||2012|
Potential role for Pex19p in assembly of PTS-receptor docking complexes.
PA3-033 was used in immunoprecipitation to investigate the role of Pex19p during the assembly of PTS-receptor docking complexes.
|Fransen M,Vastiau I,Brees C,Brys V,Mannaerts GP,Van Veldhoven PP||The Journal of biological chemistry (279:12615)||2004|