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Cytoglobin Monoclonal Antibody (4C7E11)

View all (21) Cytoglobin antibodies

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Cytoglobin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytoglobin antibody (Product # 60228-1-IG, Clone: 4C7E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Cytoglobin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 60228-1-IG (Cytoglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Cytoglobin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human bladder using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

Cytoglobin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human bladder using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

Cytoglobin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

Cytoglobin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart using 60228-1-Ig (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

Cytoglobin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 60228-1-IG (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

Cytoglobin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver using 60228-1-IG (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

Cytoglobin Antibody in Western Blot (WB) — Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytoglobin Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-IG (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytoglobin Antibody in Western Blot (WB) — Rat heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Cytoglobin Antibody in Western Blot (WB) — Pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Cytoglobin Antibody in Western Blot (WB) — Pig heart tissue were subjected to SDS PAGE followed by western blot with 60228-1-Ig (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

60228-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4C7E11

Immunogen

Cytoglobin Fusion Protein Ag4161 (1-190 aa encoded by BC029798)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MEKVPGEME IERRERSEEL SEAERKAVQA MWARLYASCE DVGVAILVRF FVNFPSAKQY FSQFKHMEDP LEMERSPQLR KHACRVMGAL NTVVENLHDP DKVSSVLALV GKAHALKHKV EPVYFKILSG VILEVVAEEF ASDFPPETQR AWAKLRGLIY SHVTAAYKEV GWVQQVPNAT TPPATLPSSG P (1-190 aa encoded by BC029798)

Target Information

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma.

Cell death & disease

Zhou P,Zhang C,Song X,Zhang D,Zhu M,Zheng H

Sat Apr 09 00:00:00 EDT 2022

ΔNp63α promotes Bortezomib resistance via the CYGB-ROS axis in head and neck squamous cell carcinoma.

Cell death & disease

Zhou P,Zhang C,Song X,Zhang D,Zhu M,Zheng H

Sat Apr 09 00:00:00 EDT 2022

Cytoglobin promotes sensitivity to ferroptosis by regulating p53-YAP1 axis in colon cancer cells.

Journal of cellular and molecular medicine

Ye S,Xu M,Zhu T,Chen J,Shi S,Jiang H,Zheng Q,Liao Q,Ding X,Xi Y

Thu Apr 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Cytoglobin; HGb; Histoglobin; stellate cell activation associated protein; Stellate cell activation-associated protein

Gene Aliases: 3110001K20Rik; CYGB; HGB; Staap; STAP

UniProt ID: (Human) Q8WWM9, (Mouse) Q9CX80, (Rat) Q921A4

Entrez Gene ID: (Pig) 100518861, (Human) 114757, (Mouse) 114886, (Rat) 170520

transport response to oxidative stress negative regulation of fibroblast migration oxygen transport fatty acid oxidation negative regulation of collagen biosynthetic process oxidation-reduction process negative regulation of hepatic stellate cell activation response to hypoxia regulation of nitric-oxide synthase activity cellular oxidant detoxification

Process(es)

catalase activity peroxidase activity oxygen transporter activity iron ion binding protein binding nitric oxide dioxygenase activity oxygen binding heme binding fatty acid peroxidase activity metal ion binding