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Cytohesin 1/2 Antibody (MA1-062) in ICC/IF
Immunofluorescent analysis of Cytohesin 1, 2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1, 2 monoclonal antibody (Product # MA1-062) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cytohesin 1, 2 staining (... View More
Product Details
MA1-062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6G11
Immunogen
Purified, His-tagged cytohesin-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
PEG precipitation
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2089016
Product Specific Information
MA1-062 detects recombinant human and mouse cytohesin-1 and 2 (ARNO).
MA1-062 has been successfully used in Western blot, IF and immunoprecipitation procedures. By Western blot, this antibody detects an ~48 kDa band representing both cytohesin-1 and cytohesin-2. In immunoprecipitation procedures MA1-062 only precipitates cytohesin-2.
The MA1-062 immunogen is purified, His-tagged cytohesin-1.
Target Information
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARF exchange factor; ARF nucleotide-binding site opener; B2-1/ARNO; CLM1; CLM2; CTC-273B12.8; cytoadhesin 1; Cytohesin-1; Cytohesin-2; homolog of secretory protein SEC7; mSec7-1; mSec7-2; PH, SEC7 and coiled-coil domain-containing protein 1; PH, SEC7 and coiled-coil domain-containing protein 2; pleckstrin homology, Sec7 and coiled-coil domains 1; pleckstrin homology, Sec7 and coiled-coil domains 2; pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2); pleckstrin homology, Sec7 and coiled/coil domains 2 (cytohesin-2); Protein ARNO; SEC7 homolog A; SEC7 homolog B; SEC7 homolog B2-1
Gene Aliases: ARNO; B2-1; CLM1; CLM2; CTH-1; CTS18; CTS18.1; CYTH1; CYTH2; CYTIP; CYTOHESIN-1; D17S811E; mKIAA4240; PSCD1; PSCD2; PSCD2L; SEC7; Sec7b; SEC7L; Sec7p-L; Sec7p-like
UniProt ID: (Human) Q15438, (Human) Q99418, (Mouse) Q9QX11, (Mouse) P63034
Entrez Gene ID: (Human) 9267, (Human) 9266, (Mouse) 19157, (Mouse) 19158
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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