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Immunofluorescent analysis of Cytohesin 1,2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody (Product # MA1-062) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, His-tagged cytohesin-1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-062 detects recombinant human and mouse cytohesin-1 and 2 (ARNO).
MA1-062 has been successfully used in Western blot, IF and immunoprecipitation procedures. By Western blot, this antibody detects an ~48 kDa band representing both cytohesin-1 and cytohesin-2. In immunoprecipitation procedures MA1-062 only precipitates cytohesin-2.
The MA1-062 immunogen is purified, His-tagged cytohesin-1.
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. The encoded protein is 83% homologous to that of CYTH2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ARF exchange factor; ARF nucleotide-binding site opener; ARNO; B2-1/ARNO; CTC-273B12.8; CYTH1; Cyth2; cytoadhesin 1; cytohesin-1; cytohesin-2; D17S811E; homolog of secretory protein SEC7; mSec7-1; mSec7-2; PH SEC7 and coiled-coil domain-containing protein 2; PH, SEC7 and coiled-coil domain-containing protein 1; PH, SEC7 and coiled-coil domain-containing protein 2; pleckstrin homology, Sec7 and coiled-coil domains 1; pleckstrin homology, Sec7 and coiled-coil domains 2; pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2); pleckstrin homology, Sec7 and coiled/coil domains 2 (cytohesin-2); Protein ARNO; PSCD1; PSCD2; PSCD2L; SEC7 and coiled-coil domain-containing protein 1; SEC7 homolog A; SEC7 homolog B; SEC7 homolog B2-1
ARNO; B2-1; CLM1; CLM2; CTH-1; CTS18; CTS18.1; CYTH1; CYTH2; CYTIP; CYTOHESIN-1; D17S811E; mKIAA4240; PSCD1; PSCD2; PSCD2L; SEC7; Sec7b; SEC7L; Sec7p-L; Sec7p-like