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Cytohesin 4 Antibody (PA5-120313) in WB
Western blot analysis of Cytohesin 4 in extracts of various cell lines. Samples were incubated with Cytohesin 4 Polyclonal antibody (Product # PA5-120313) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-120313
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human CYTH4 (NP_037517.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.85 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913885
Product Specific Information
Positive test controls include: Mouse liver, Mouse brain, Rat brain. The target is usually found in the following locations: Cell membrane, Peripheral membrane protein.
Immunogen sequence: MDLCHPEPAE LSSGETEELQ RIKWHRKQLL EDIQKLKDEI ADVFAQIDCF ESAEESRMAQ KEKELCIGRK
Target Information
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with both ARF1 and ARF5 but is inactive with ARF6. The structures of this gene and CYTH1 are very similar.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytohesin-4; PH, SEC7 and coiled-coil domain-containing protein 4; pleckstrin homology, Sec7 and coiled/coil domains 4
Gene Aliases: 2510004M07Rik; 5830469K17Rik; AI467541; Cyth4; mFLJ00017; Pscd4; RGD1564842
UniProt ID: (Mouse) Q80YW0
Entrez Gene ID: (Mouse) 72318, (Rat) 500906
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