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DE-K13 reacts with cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with Cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. Cytokeratin 10 is present in Keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while Cytokeratin 13 is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia, as well as carcinomas derived from these tissues.
Cross-reactivity: Not reactive in mouse and rat.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Cytokeratins(CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie. Skin) and non-keratinizing cells (ie. Mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different CKs in the molecular range of 40-70 k Da and isoelectric points of 5-805 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CK-10; CK-13; cytokeratin 10; cytokeratin 13; Cytokeratin-10; Cytokeratin-13; epithelial keratin 10; Epithelial keratin-10; K10; K13; keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris); keratin 10, type I; keratin 13, type I; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 13; Keratin-10; Keratin-13
Gene Aliases: BCIE; BIE; CK-10; CK10; CK13; EHK; K10; K13; KER10; KPP; KRT10; KRT13; WSN2
UniProt ID: (Human) P13645, (Dog) Q6EIZ0, (Human) P13646
Entrez Gene ID: (Human) 3858, (Dog) 491006, (Dog) 490983, (Human) 3860
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