|Tested species reactivity||Dog, Cat, Human, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeletal preparation extracted from human ectocervical epithelium|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13702 targets Cytokeratin 10 in IF, IHC (P), and WB applications and shows reactivity with Canine, Feline, Human, and Rat samples.
The MA5-13702 immunogen is cytoskeletal preparation extracted from human ectocervical epithelium.
CK10 (Cytokeratin 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appeares in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Barrier properties of an N/TERT-based human skin equivalent.
MA5-13702 was used in immunohistochemistry to evaluate the barrier properties of human skin equivalents created using the N/TERT keratinocyte cell line
|van Drongelen V,Danso MO,Mulder A,Mieremet A,van Smeden J,Bouwstra JA,El Ghalbzouri A||Tissue engineering. Part A (20:3041)||2014|
HIV nucleoside reverse transcriptase inhibitors efavirenz and tenofovir change the growth and differentiation of primary gingival epithelium.
MA5-13702 was used in immunohistochemistry to use organotypic gingival keratinocyte culture to explore the effects of HIV nucleoside reverse transcriptase inhibitors on the gingival epithelium
|Mitchell D,Israr M,Alam S,Dinello D,Kishel J,Jia R,Meyers C||HIV medicine (15:196)||2014|
Inflammatory and antimicrobial responses to methicillin-resistant Staphylococcus aureus in an in vitro wound infection model.
MA5-13702 was used in immunohistochemistry to develop an in vitro model of thermal wound infection using human skin equivalents in order to investigate Staphylococcus wound colonization
|Haisma EM,Rietveld MH,de Breij A,van Dissel JT,El Ghalbzouri A,Nibbering PH||PloS one (8:null)||2013|
Integrated experimental and computational approach to understand the effects of heavy ion radiation on skin homeostasis.
MA5-13702 was used in immunohistochemistry to study the effects of heavy ion radiation on skin metabolism using both experimental and in silico approaches
|von Neubeck C,Shankaran H,Geniza MJ,Kauer PM,Robinson RJ,Chrisler WB,Sowa MB||Integrative biology : quantitative biosciences from nano to macro (5:1229)||2013|
The expression profiles of acidic epithelial keratins in ameloblastoma.
MA5-13702 was used in immunohistochemistry to study the expression of 9 acidic keratins in ameloblastoma
|Pal SK,Sakamoto K,Aragaki T,Akashi T,Yamaguchi A||Oral surgery, oral medicine, oral pathology and oral radiology (115:523)||2013|
Control of somatic tissue differentiation by the long non-coding RNA TINCR.
MA5-13702 was used in immunohistochemistry to study the role of the long non-coding RNA TINCR in somatic cell differentiation
|Kretz M,Siprashvili Z,Chu C,Webster DE,Zehnder A,Qu K,Lee CS,Flockhart RJ,Groff AF,Chow J,Johnston D,Kim GE,Spitale RC,Flynn RA,Zheng GX,Aiyer S,Raj A,Rinn JL,Chang HY,Khavari PA||Nature (493:231)||2013|
Aberrant keratinization of reticular oral lichen planus is related to elastolysis.
MA5-13702 was used in immunohistochemistry to study the role of elastases in the defective keratinization observed in oral lichen planus
|Liao SC,Hsieh PC,Huang JS,Hsu CW,Yuan K||Oral surgery, oral medicine, oral pathology and oral radiology (113:808)||2012|
Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium.
MA5-13702 was used in immunohistochemistry to investigate the influence of zidovudine on gingival epithelium growth
|Mitchell D,Israr M,Alam S,Kishel J,Dinello D,Meyers C||HIV medicine (13:276)||2012|
Thymic microenvironment reconstitution after postnatal human thymus transplantation.
MA5-13702 was used in immunohistochemistry to investigate thymus development after postnatal thymus transplantation
|Li B,Li J,Devlin BH,Markert ML||Clinical immunology (Orlando, Fla.) (140:244)||2011|
Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis.
MA5-13702 was used in immunohistochemistry to study the expression of keratin 4 and keratin 13 in oral squamous cell carcinoma and epithelial dysplasia
|Sakamoto K,Aragaki T,Morita K,Kawachi H,Kayamori K,Nakanishi S,Omura K,Miki Y,Okada N,Katsube K,Takizawa T,Yamaguchi A||Histopathology (58:531)||2011|
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
MA5-13702 was used in immunohistochemistry to examine the epithelial differentiation of human embryonic stem cells
|Metallo CM,Azarin SM,Moses LE,Ji L,de Pablo JJ,Palecek SP||Tissue engineering. Part A (16:213)||2010|
Cadherin-mediated cell-cell contact regulates keratinocyte differentiation.
MA5-13702 was used in immunohistochemistry to study the role of cadherin-mediated cell-cell contact in regulating keratinocyte differentiation
|Charest JL,Jennings JM,King WP,Kowalczyk AP,García AJ||The Journal of investigative dermatology (129:564)||2009|
Use of allograft biopsies to assess thymopoiesis after thymus transplantation.
MA5-13702 was used in immunohistochemistry to evaluate the biopsy method to assess thymopoiesis after thymus transplantation
|Markert ML,Li J,Devlin BH,Hoehner JC,Rice HE,Skinner MA,Li YJ,Hale LP||Journal of immunology (Baltimore, Md. : 1950) (180:6354)||2008|
An altered keratinocyte phenotype in oral submucous fibrosis: correlation of keratin K17 expression with disease severity.
MA5-13702 was used in immunohistochemistry to study keratin expression and keratinocyte phenotype in oral submucous fibrosis
|Lalli A,Tilakaratne WM,Ariyawardana A,Fitchett C,Leigh IM,Hagi-Pavli E,Cruchley AT,Parkinson EK,Teh MT,Fortune F,Waseem A||Journal of oral pathology and medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (37:211)||2008|
|Not Applicable||Not Cited||
Adenosquamous carcinoma with cilium formation, mucin production and keratinization in the nasal cavity of a red fox (Vulpes vulpes schrencki).
MA5-13702 was used in immunohistochemistry to report on a case of adenosquamous carcinoma in the nasal cavity of a red fox
|Fukui D,Bando G,Ishikawa Y,Kadota K||Journal of comparative pathology (137:142)||2007|
A study of cytokeratin profiles in localized cutaneous amyloids.
MA5-13702 was used in immunohistochemistry to investigate the expression profile of cytokeratins in cutaneous amyloids
|Chang YT,Liu HN,Wang WJ,Lee DD,Tsai SF||Archives of dermatological research (296:83)||2004|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA5-13702 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
MA5-13702 was used in immunohistochemistry to study the regulation of epithelial cell differentiation and human thymic medulla Hassall body formation by corticosteroids
|Hale LP,Markert ML||Journal of immunology (Baltimore, Md. : 1950) (172:617)||2004|
Molecular profiling of bladder cancer using cDNA microarrays: defining histogenesis and biological phenotypes.
MA5-13702 was used in immunohistochemistry to study the molecular profile of bladder cancer using cDNA microarrays
|Sanchez-Carbayo M,Socci ND,Charytonowicz E,Lu M,Prystowsky M,Childs G,Cordon-Cardo C||Cancer research (62:6973)||2002|
Quantitative phosphoproteomics identifies filaggrin and other targets of ionizing radiation in a human skin model.
MA5-13702 was used in western blot to identify targets of ionizing radiation in a human skin tissue model using a quantitative phospho-proteomic approach
|Yang F,Waters KM,Webb-Robertson BJ,Sowa MB,von Neubeck C,Aldrich JT,Markillie LM,Wirgau RM,Gritsenko MA,Zhao R,Camp DG,Smith RD,Stenoien DL||Experimental dermatology (21:352)||2012|
Plakin proteins are coordinately cleaved during apoptosis but preferentially through the action of different caspases.
MA5-13702 was used in western blot to study the role of different caspases in the coordinate cleavage of plakin proteins during apoptosis
|Aho S||Experimental dermatology (13:700)||2004|
B-cell maturation antigen (BCMA) activation exerts specific proinflammatory effects in normal human keratinocytes and is preferentially expressed in inflammatory skin pathologies.
MA5-13702 was used in immunocytochemistry to study the expression and role of B-cell maturation antigen in inflammatory skin pathologies
|Alexaki VI,Pelekanou V,Notas G,Venihaki M,Kampa M,Dessirier V,Sabour-Alaoui S,Stathopoulos EN,Tsapis A,Castanas E||Endocrinology (153:739)||2012|
Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes.
MA5-13702 was used in immunocytochemistry and western blot to study the role of homeodomain-only protein HOP as a novel modulator of late differentiation in keratinocytes
|Obarzanek-Fojt M,Favre B,Kypriotou M,Ryser S,Huber M,Hohl D||European journal of cell biology (90:279)||2011|
Induction of p38, tumour necrosis factor-¿ and RANTES by mechanical stretching of keratinocytes expressing mutant keratin 10R156H.
MA5-13702 was used in immunocytochemistry and western blot to study the induction of p38, tumour necrosis factor-? and RANTES by mechanical stretching of keratinocytes expressing mutant keratin 10R156H
|Obarzanek-Fojt M,Favre B,Huber M,Ryser S,Moodycliffe AM,Wipff PJ,Hinz B,Hohl D||The British journal of dermatology (164:125)||2011|