|Tested species reactivity||Dog, Cat, Human, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeletal preparation extracted from human ectocervical epithelium|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13705 targets Cytokeratin 10 in IF, IHC (P), and WB applications and shows reactivity with Canine, Feline, Human, and Rat samples.
The MA5-13705 immunogen is cytoskeletal preparation extracted from human ectocervical epithelium.
CK10 (Cytokeratin 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appeares in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cx26 knockout predisposes the mammary gland to primary mammary tumors in a DMBA-induced mouse model of breast cancer.
MA5-13705 was used in immunohistochemistry - paraffin section to discuss Cx26 knockout in DMBA-induced mouse models and breast cancer
|Stewart MK,Bechberger JF,Welch I,Naus CC,Laird DW||Oncotarget (6:37185)||2015|
The effect of low dose ionizing radiation on homeostasis and functional integrity in an organotypic human skin model.
MA5-13705 was used in immunohistochemistry - paraffin section to use an in vitro three dimensional human organotypic skin tissue model to examine the effects of low doses of high linear energy transfer oxygen, silicon, and iron ions.
|von Neubeck C,Geniza MJ,Kauer PM,Robinson RJ,Chrisler WB,Sowa MB||Mutation research (775:10)||2015|
Immunohistochemical LRIG3 expression in cervical intraepithelial neoplasia and invasive squamous cell cervical cancer: association with expression of tumor markers, hormones, high-risk HPV-infection, smoking and patient outcome.
MA5-13705 was used in immunohistochemistry - paraffin section to analyze LRIG3 expression through immunohistochemical studies in cervical intraepithelial neoplasia and invasive squamous cell cervical cancer and the association with tumor markers, hormones
|Lindström AK,Hellberg D||European journal of histochemistry : EJH (58:null)||2014|
Tissue tumor marker expression in smokers, including serum cotinine concentrations, in women with cervical intraepithelial neoplasia or normal squamous cervical epithelium.
MA5-13705 was used in immunohistochemistry - paraffin section to study tissue tumor marker expression in smokers with cervical intraepithelial neoplasia or normal squamous cervical epithelium
|Samir R,Asplund A,Tot T,Pekar G,Hellberg D||American journal of obstetrics and gynecology (202:579.e1)||2010|
Development of full-thickness human skin equivalents with blood and lymph-like capillary networks by cell coating technology.
MA5-13705 was used in immunohistochemistry to develop a 3D human skin equivalent containing blood and lymph-like capillary networks
|Matsusaki M,Fujimoto K,Shirakata Y,Hirakawa S,Hashimoto K,Akashi M||Journal of biomedical materials research. Part A (103:3386)||2015|
Barrier properties of an N/TERT-based human skin equivalent.
MA5-13705 was used in immunohistochemistry to evaluate the barrier properties of human skin equivalents created using the N/TERT keratinocyte cell line
|van Drongelen V,Danso MO,Mulder A,Mieremet A,van Smeden J,Bouwstra JA,El Ghalbzouri A||Tissue engineering. Part A (20:3041)||2014|
WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis.
MA5-13705 was used in immunohistochemistry to establish an in vitro system to study three-dimensional corneal differentiation
|Ouyang H,Xue Y,Lin Y,Zhang X,Xi L,Patel S,Cai H,Luo J,Zhang M,Zhang M,Yang Y,Li G,Li H,Jiang W,Yeh E,Lin J,Pei M,Zhu J,Cao G,Zhang L,Yu B,Chen S,Fu XD,Liu Y,Zhang K||Nature (511:358)||2014|
HIV nucleoside reverse transcriptase inhibitors efavirenz and tenofovir change the growth and differentiation of primary gingival epithelium.
MA5-13705 was used in immunohistochemistry to use organotypic gingival keratinocyte culture to explore the effects of HIV nucleoside reverse transcriptase inhibitors on the gingival epithelium
|Mitchell D,Israr M,Alam S,Dinello D,Kishel J,Jia R,Meyers C||HIV medicine (15:196)||2014|
Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis.
MA5-13705 was used in immunohistochemistry to study the effects of conditional epidermal knockout of HDAC-1 and -2 on murine ectodermal organ morphogenesis
|Hughes MW,Jiang TX,Lin SJ,Leung Y,Kobielak K,Widelitz RB,Chuong CM||The Journal of investigative dermatology (134:24)||2014|
Inflammatory and antimicrobial responses to methicillin-resistant Staphylococcus aureus in an in vitro wound infection model.
MA5-13705 was used in immunohistochemistry to develop an in vitro model of thermal wound infection using human skin equivalents in order to investigate Staphylococcus wound colonization
|Haisma EM,Rietveld MH,de Breij A,van Dissel JT,El Ghalbzouri A,Nibbering PH||PloS one (8:null)||2013|
Integrated experimental and computational approach to understand the effects of heavy ion radiation on skin homeostasis.
MA5-13705 was used in immunohistochemistry to study the effects of heavy ion radiation on skin metabolism using both experimental and in silico approaches
|von Neubeck C,Shankaran H,Geniza MJ,Kauer PM,Robinson RJ,Chrisler WB,Sowa MB||Integrative biology : quantitative biosciences from nano to macro (5:1229)||2013|
The expression profiles of acidic epithelial keratins in ameloblastoma.
MA5-13705 was used in immunohistochemistry to study the expression of 9 acidic keratins in ameloblastoma
|Pal SK,Sakamoto K,Aragaki T,Akashi T,Yamaguchi A||Oral surgery, oral medicine, oral pathology and oral radiology (115:523)||2013|
Control of somatic tissue differentiation by the long non-coding RNA TINCR.
MA5-13705 was used in immunohistochemistry to study the role of the long non-coding RNA TINCR in somatic cell differentiation
|Kretz M,Siprashvili Z,Chu C,Webster DE,Zehnder A,Qu K,Lee CS,Flockhart RJ,Groff AF,Chow J,Johnston D,Kim GE,Spitale RC,Flynn RA,Zheng GX,Aiyer S,Raj A,Rinn JL,Chang HY,Khavari PA||Nature (493:231)||2013|
Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium.
MA5-13705 was used in immunohistochemistry to investigate the influence of zidovudine on gingival epithelium growth
|Mitchell D,Israr M,Alam S,Kishel J,Dinello D,Meyers C||HIV medicine (13:276)||2012|
Thymic microenvironment reconstitution after postnatal human thymus transplantation.
MA5-13705 was used in immunohistochemistry to investigate thymus development after postnatal thymus transplantation
|Li B,Li J,Devlin BH,Markert ML||Clinical immunology (Orlando, Fla.) (140:244)||2011|
Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis.
MA5-13705 was used in immunohistochemistry to study the expression of keratin 4 and keratin 13 in oral squamous cell carcinoma and epithelial dysplasia
|Sakamoto K,Aragaki T,Morita K,Kawachi H,Kayamori K,Nakanishi S,Omura K,Miki Y,Okada N,Katsube K,Takizawa T,Yamaguchi A||Histopathology (58:531)||2011|
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
MA5-13705 was used in immunohistochemistry to examine the epithelial differentiation of human embryonic stem cells
|Metallo CM,Azarin SM,Moses LE,Ji L,de Pablo JJ,Palecek SP||Tissue engineering. Part A (16:213)||2010|
Cadherin-mediated cell-cell contact regulates keratinocyte differentiation.
MA5-13705 was used in immunohistochemistry to study the role of cadherin-mediated cell-cell contact in regulating keratinocyte differentiation
|Charest JL,Jennings JM,King WP,Kowalczyk AP,García AJ||The Journal of investigative dermatology (129:564)||2009|
Use of allograft biopsies to assess thymopoiesis after thymus transplantation.
MA5-13705 was used in immunohistochemistry to evaluate the biopsy method to assess thymopoiesis after thymus transplantation
|Markert ML,Li J,Devlin BH,Hoehner JC,Rice HE,Skinner MA,Li YJ,Hale LP||Journal of immunology (Baltimore, Md. : 1950) (180:6354)||2008|
An altered keratinocyte phenotype in oral submucous fibrosis: correlation of keratin K17 expression with disease severity.
MA5-13705 was used in immunohistochemistry to study keratin expression and keratinocyte phenotype in oral submucous fibrosis
|Lalli A,Tilakaratne WM,Ariyawardana A,Fitchett C,Leigh IM,Hagi-Pavli E,Cruchley AT,Parkinson EK,Teh MT,Fortune F,Waseem A||Journal of oral pathology and medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (37:211)||2008|
|Not Applicable||Not Cited||
Adenosquamous carcinoma with cilium formation, mucin production and keratinization in the nasal cavity of a red fox (Vulpes vulpes schrencki).
MA5-13705 was used in immunohistochemistry to report on a case of adenosquamous carcinoma in the nasal cavity of a red fox
|Fukui D,Bando G,Ishikawa Y,Kadota K||Journal of comparative pathology (137:142)||2007|
A study of cytokeratin profiles in localized cutaneous amyloids.
MA5-13705 was used in immunohistochemistry to investigate the expression profile of cytokeratins in cutaneous amyloids
|Chang YT,Liu HN,Wang WJ,Lee DD,Tsai SF||Archives of dermatological research (296:83)||2004|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA5-13705 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
MA5-13705 was used in immunohistochemistry to study the regulation of epithelial cell differentiation and human thymic medulla Hassall body formation by corticosteroids
|Hale LP,Markert ML||Journal of immunology (Baltimore, Md. : 1950) (172:617)||2004|
Molecular profiling of bladder cancer using cDNA microarrays: defining histogenesis and biological phenotypes.
MA5-13705 was used in immunohistochemistry to study the molecular profile of bladder cancer using cDNA microarrays
|Sanchez-Carbayo M,Socci ND,Charytonowicz E,Lu M,Prystowsky M,Childs G,Cordon-Cardo C||Cancer research (62:6973)||2002|
Expression and localization of epithelial stem cell and differentiation markers in equine skin, eye and hoof.
MA5-13705 was used in immunohistochemistry - paraffin section and western blot to characterize localization and expression of epithelial stem cell and differentiation markers in equine eye, hoof, and skin
|Linardi RL,Megee SO,Mainardi SR,Senoo M,Galantino-Homer HL||Veterinary dermatology (26:213)||2015|
The ¿-opioid receptor affects epidermal homeostasis via ERK-dependent inhibition of transcription factor POU2F3.
MA5-13705 was used in western blot to investigate the role of delta-opioid receptor in human keratinocytes
|Neumann C,Bigliardi-Qi M,Widmann C,Bigliardi PL||The Journal of investigative dermatology (135:471)||2015|
Quantitative phosphoproteomics identifies filaggrin and other targets of ionizing radiation in a human skin model.
MA5-13705 was used in western blot to identify targets of ionizing radiation in a human skin tissue model using a quantitative phospho-proteomic approach
|Yang F,Waters KM,Webb-Robertson BJ,Sowa MB,von Neubeck C,Aldrich JT,Markillie LM,Wirgau RM,Gritsenko MA,Zhao R,Camp DG,Smith RD,Stenoien DL||Experimental dermatology (21:352)||2012|
Plakin proteins are coordinately cleaved during apoptosis but preferentially through the action of different caspases.
MA5-13705 was used in western blot to study the role of different caspases in the coordinate cleavage of plakin proteins during apoptosis
|Aho S||Experimental dermatology (13:700)||2004|
Phenotypic plasticity in normal breast derived epithelial cells.
MA5-13705 was used in immunocytochemistry to characterize primary, non-transformed breast epithelial cells by immunohistochemistry, flow cytometry, and in vitro cell culture
|Sauder CA,Koziel JE,Choi M,Fox MJ,Grimes BR,Badve S,Blosser RJ,Radovich M,Lam CC,Vaughan MB,Herbert BS,Clare SE||BMC cell biology (15:null)||2014|
A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells.
MA5-13705 was used in immunocytochemistry to describe conditions that promote development of hPSC-derived simple epithelial cells
|Lian X,Selekman J,Bao X,Hsiao C,Zhu K,Palecek SP||PloS one (8:null)||2013|
B-cell maturation antigen (BCMA) activation exerts specific proinflammatory effects in normal human keratinocytes and is preferentially expressed in inflammatory skin pathologies.
MA5-13705 was used in immunocytochemistry to study the expression and role of B-cell maturation antigen in inflammatory skin pathologies
|Alexaki VI,Pelekanou V,Notas G,Venihaki M,Kampa M,Dessirier V,Sabour-Alaoui S,Stathopoulos EN,Tsapis A,Castanas E||Endocrinology (153:739)||2012|
Induction of p38, tumour necrosis factor-¿ and RANTES by mechanical stretching of keratinocytes expressing mutant keratin 10R156H.
MA5-13705 was used in immunocytochemistry and western blot to study the induction of p38, tumour necrosis factor-? and RANTES by mechanical stretching of keratinocytes expressing mutant keratin 10R156H
|Obarzanek-Fojt M,Favre B,Huber M,Ryser S,Moodycliffe AM,Wipff PJ,Hinz B,Hohl D||The British journal of dermatology (164:125)||2011|