|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytokeratin preparation extracted from human esophagus.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA1-06322 detects cytokeratin 13 in human samples.
MA1-06322 has sucessfully been used in Western blotting, and immunohistochemistry.
The MA1-06322 immunogen is cytokeratin extracted from human esophagus cells.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The two faces of cervical adenocarcinoma in situ.
MA1-06322 was used in immunohistochemistry to investigate the expression of multiple proteins in different types of premalignant lesions of the uterine cervix
|Smedts F,Ramaekers FC,Hopman AH||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (29:378)||2010|
Keratin profiling in the developing human prostate. A different approach to understanding epithelial lineage.
MA1-06322 was used in immunohistochemistry to investigate the distribution of keratin in prostate during development
|Trompetter M,Smedts F,van der Wijk J,Schoots C,de Jong HJ,Hopman A,de la Rosette J||Anticancer research (28:237)||2008|