|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Sheep, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from C-terminal of human Keratin 14|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400-1:800|
|Western Blot (WB)||1 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 6 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
PA5-16722 targets Cytokeratin 14 in immunohistochemistry (paraffin and Western blot applications and shows reactivity with Human samples.
The PA5-16722 immunogen is a synthetic peptide derived from C-terminal of human Keratin 14.
Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5. Keratin 14 has been studied as a prognostic marker in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Programmed synthesis of three-dimensional tissues.
PA5-16722 was used in immunocytochemistry to study three-dimensional tissues by programmed synthesis
|Todhunter ME,Jee NY,Hughes AJ,Coyle MC,Cerchiari A,Farlow J,Garbe JC,LaBarge MA,Desai TA,Gartner ZJ||Nature methods (12:975)||2015|
Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias.
PA5-16722 was used in immunocytochemistry to study and identify critical cell lineage-dependent transduction bias to model breast cancer properly
|Hines WC,Yaswen P,Bissell MJ||Nature communications (6:null)||2015|
Py2T murine breast cancer cells, a versatile model of TGFß-induced EMT in vitro and in vivo.
PA5-16722 was used in immunocytochemistry to develop a novel cellular model of epithelial-to-mesenchymal transition based on the use of mouse Py2T breast cancer cells
|Waldmeier L,Meyer-Schaller N,Diepenbruck M,Christofori G||PloS one (7:null)||2012|
An immunohistochemical method to study breast cancer cell subpopulations and their growth regulation by hormones in three-dimensional cultures.
PA5-16722 was used in immunocytochemistry and immunohistochemistry - paraffin section to develop a simple and inexpensive method to analyze cell subpopulations in mixed-cell colonies
|Pinto MP,Jacobsen BM,Horwitz KB||Frontiers in endocrinology (2:null)||2012|
PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours.
PA5-16722 was used in western blot to elucidate the potential of PIK3CA to induce multipotency during tumorigenesis in the mammary gland.
|Koren S,Reavie L,Couto JP,De Silva D,Stadler MB,Roloff T,Britschgi A,Eichlisberger T,Kohler H,Aina O,Cardiff RD,Bentires-Alj M||Nature (525:114)||2015|
Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition.
PA5-16722 was used in western blot to evaluate the effect of aspirin in mesenchymal to epithelial transition
|Maity G,De A,Das A,Banerjee S,Sarkar S,Banerjee SK||Laboratory investigation; a journal of technical methods and pathology (95:702)||2015|
Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
PA5-16722 was used in immunohistochemistry - paraffin section to investigate the role of afadin in epithelial tissue development using epithelium-specific afadin-deficient mice
|Yoshida T,Iwata T,Takai Y,Birchmeier W,Yamato M,Okano T||Genes to cells : devoted to molecular and cellular mechanisms (19:842)||2014|
Solitary tumours associated with Jaagsiekte retrovirus in sheep are heterogeneous and contain cells expressing markers identifying progenitor cells in lung repair.
PA5-16722 was used in immunohistochemistry to study the heterogeneity and the presence of lung repair progenitor cells in Jaagsiekte retrovirus-associated solitary tumors in sheep
|De las Heras M,de Martino A,Borobia M,Ortín A,Álvarez R,Borderías L,Giménez-Más JA||Journal of comparative pathology (150:138)||2014|
Label-retaining, quiescent globose basal cells are found in the olfactory epithelium.
PA5-16722 was used in immunohistochemistry to study the presence in olfactory epithelium of quiescent globose basal cells that retain thymidine analogue labels
|Jang W,Chen X,Flis D,Harris M,Schwob JE||The Journal of comparative neurology (522:731)||2014|
Global expression profiling of globose basal cells and neurogenic progression within the olfactory epithelium.
PA5-16722 was used in immunohistochemistry to profile the expression pattern of globose basal cells within the olfactory epithelium
|Krolewski RC,Packard A,Schwob JE||The Journal of comparative neurology (521:833)||2013|
Ascl1 (Mash1) knockout perturbs differentiation of nonneuronal cells in olfactory epithelium.
PA5-16722 was used in immunohistochemistry to study the abnormal differentiation of non-neuronal cells in the olfactory epithelium of ASCL1 knockout mice
|Krolewski RC,Packard A,Jang W,Wildner H,Schwob JE||PloS one (7:null)||2013|
Luminal expression of PIK3CA mutant H1047R in the mammary gland induces heterogeneous tumors.
PA5-16722 was used in immunohistochemistry to elucidate the contribution of PIK3CA H1047R to breast cancer pathogenesis.
|Meyer DS,Brinkhaus H,Müller U,Müller M,Cardiff RD,Bentires-Alj M||Cancer research (71:4344)||2011|
|Not Applicable||Not Cited||
An oestrogen-dependent model of breast cancer created by transformation of normal human mammary epithelial cells.
PA5-16722 was used in immunocytochemistry and immunohistochemistry to develop an estrogen receptor-alpha-positive model of breast cancer using human mammary epithelial cells
|Duss S,André S,Nicoulaz AL,Fiche M,Bonnefoi H,Brisken C,Iggo RD||Breast cancer research : BCR (9:null)||2007|
A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells.
PA5-16722 was used in flow cytometry and immunocytochemistry to describe conditions that promote development of hPSC-derived simple epithelial cells
|Lian X,Selekman J,Bao X,Hsiao C,Zhu K,Palecek SP||PloS one (8:null)||2013|