Immunofluorescent analysis of Cytokeratin 15 (green) showing staining in the cytoplasm of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytokeratin 15 monoclonal antibody (Product # MA5-11344) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Dog, Yeast, Sheep, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11344 targets Cytokeratin 15 in ICC/IF, IHC-P and Western blot applications and shows reactivity with Bovine, Human, mouse, Non-human primate and Rat samples.
The MA5-11344 immunogen is a 17-mer synthetic peptide from the C-terminus of human cytokeratin 15.
Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture.
MA5-11344 was used in immunocytochemistry to analyze propagation of oestrogen-responsive and oestrogen receptor-positive normal human breast cells in culture
|Fridriksdottir AJ,Kim J,Villadsen R,Klitgaard MC,Hopkinson BM,Petersen OW,Rønnov-Jessen L||Nature communications (6:null)||2015|
Lineage tracing of hair follicle stem cells in epidermal whole mounts.
MA5-11344 was used in immunocytochemistry to describe a protocol that enables the labeling and tracking of murine hair follicle bulge stem cells and determination of their fates
|Petersson M,Frances D,Niemann C||Methods in molecular biology (Clifton, N.J.) (989:45)||2013|
An improved method of human keratinocyte culture from skin explants: cell expansion is linked to markers of activated progenitor cells.
MA5-11344 was used in immunocytochemistry to investigate the effectivenss of a novel method for human keratinocyte preparation
|Guo A,Jahoda CA||Experimental dermatology (18:720)||2009|
Genetic dissection of the mechanisms underlying telomere-associated diseases: impact of the TRF2 telomeric protein on mouse epidermal stem cells.
MA5-11344 was used in immunocytochemistry to study the role of the TRF2 telomere protein in telomere-associated diseases
|Stout GJ,Blasco MA||Disease models and mechanisms (2:139)||2009|
Expression of the hair stem cell-specific marker nestin in epidermal and follicular tumors.
MA5-11344 was used in immunocytochemistry to examine the pathogenesis of epidermal and follicular tumors, trichilemmoma, basal cell carcinoma, and squamous cell carcinoma
|Kanoh M,Amoh Y,Sato Y,Katsuoka K||European journal of dermatology : EJD (18:518)||2008|
Isolation and identification of stem cells from adult cashmere goat skin.
MA5-11344 was used in immunocytochemistry to examine the totipotency of adult cashmere goat skin epithelia
|Liu Y,Zhou H,Gao F||International journal of dermatology (47:551)||2008|
Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus.
MA5-11344 was used in immunocytochemistry to characterize the putative epidermal stem cells from Cashmere goat fetus
|Islam MS,Zhou H||European journal of dermatology : EJD (17:302)||2007|
Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis.
MA5-11344 was used in immunohistochemistry to study the effects of conditional epidermal knockout of HDAC-1 and -2 on murine ectodermal organ morphogenesis
|Hughes MW,Jiang TX,Lin SJ,Leung Y,Kobielak K,Widelitz RB,Chuong CM||The Journal of investigative dermatology (134:24)||2014|
Label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region define stem cells in the sweat gland.
MA5-11344 was used in immunohistochemistry to determine how label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region create stem cells in the sweat gland
|Leung Y,Kandyba E,Chen YB,Ruffins S,Kobielak K||PloS one (8:null)||2013|
Clinical, dermoscopic and immunohistochemical assessment of actinic keratoses and evaluation of the effectiveness of diclofenac therapy with immunohistochemical analysis.
MA5-11344 was used in immunohistochemistry to study the immunopathology and efficacy of diclofenac treatment in patients with actinic keratoses
|Çayirli M,Köse O,Demiriz M||Archives of dermatological research (305:389)||2013|
E2F1 loss induces spontaneous tumour development in Rb-deficient epidermis.
MA5-11344 was used in immunohistochemistry to study spontaneous tumor development in murine epidermis lacking both retinoblastoma protein and E2F-1
|Costa C,Santos M,Martínez-Fernández M,Dueñas M,Lorz C,García-Escudero R,Paramio JM||Oncogene (32:2937)||2013|
Aquaporin 1-positive stromal niche-like cells directly interact with N-cadherin-positive clusters in the basal limbal epithelium.
MA5-11344 was used in immunohistochemistry to study the interaction of aquaporin-positive stromal stromal niche-like cells with N-cadherin-positive limbal basal epithelial progenitor cells
|Higa K,Kato N,Yoshida S,Ogawa Y,Shimazaki J,Tsubota K,Shimmura S||Stem cell research (10:147)||2013|
Intraepithelial paracrine Hedgehog signaling induces the expansion of ciliated cells that express diverse progenitor cell markers in the basal epithelium of the mouse mammary gland.
MA5-11344 was used in immunohistochemistry to study the role of Hedgehog signaling in normal mammary tissue and breast cancer using a murine model
|García-Zaragoza E,Pérez-Tavarez R,Ballester A,Lafarga V,Jiménez-Reinoso A,Ramírez A,Murillas R,Gallego MI||Developmental biology (372:28)||2012|
Detailed histological structure of human hair follicle bulge region at different ages: a visible niche for nesting adult stem cells.
MA5-11344 was used in immunohistochemistry to study the structure and stem cell content of the human hair follicle bulge region using a histological approach
|Wang X,Shi Y,Zhou Q,Liu X,Xu S,Lei T||Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (32:648)||2012|
Prostaglandin D2 inhibits hair growth and is elevated in bald scalp of men with androgenetic alopecia.
MA5-11344 was used in immunohistochemistry to study the role of prostaglandin D2 in hair growth and androgenetic alopecia
|Garza LA,Liu Y,Yang Z,Alagesan B,Lawson JA,Norberg SM,Loy DE,Zhao T,Blatt HB,Stanton DC,Carrasco L,Ahluwalia G,Fischer SM,FitzGerald GA,Cotsarelis G||Science translational medicine (4:null)||2012|
Midbody accumulation through evasion of autophagy contributes to cellular reprogramming and tumorigenicity.
MA5-11344 was used in immunohistochemistry to study the role of midbody accumulation in cellular reprogramming and tumorigenicity
|Kuo TC,Chen CT,Baron D,Onder TT,Loewer S,Almeida S,Weismann CM,Xu P,Houghton JM,Gao FB,Daley GQ,Doxsey S||Nature cell biology (13:1214)||2011|
Global 5-hydroxymethylcytosine content is significantly reduced in tissue stem/progenitor cell compartments and in human cancers.
MA5-11344 was used in immunohistochemistry to study the low levels of 5-hydroxymethylcytosine observed in stem/progenitor cells and cancer cells as compared to normal tissues
|Haffner MC,Chaux A,Meeker AK,Esopi DM,Gerber J,Pellakuru LG,Toubaji A,Argani P,Iacobuzio-Donahue C,Nelson WG,Netto GJ,De Marzo AM,Yegnasubramanian S||Oncotarget (2:627)||2011|
Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells.
MA5-11344 was used in immunohistochemistry to investigate the mechanism of androgenetic alopecia pathogenesis
|Garza LA,Yang CC,Zhao T,Blatt HB,Lee M,He H,Stanton DC,Carrasco L,Spiegel JH,Tobias JW,Cotsarelis G||The Journal of clinical investigation (121:613)||2011|
Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
MA5-11344 was used in immunohistochemistry to study the expression pattern of stem cells in the bulbar conjunctival epithelium
|Qi H,Zheng X,Yuan X,Pflugfelder SC,Li DQ||Journal of cellular physiology (225:180)||2010|
A functional role of RB-dependent pathway in the control of quiescence in adult epidermal stem cells revealed by genomic profiling.
MA5-11344 was used in immunohistochemistry to investigate the mechanism for the regulation of hair follicle stem cell homeostasis
|Lorz C,García-Escudero R,Segrelles C,Garín MI,Ariza JM,Santos M,Ruiz S,Lara MF,Martínez-Cruz AB,Costa C,Buitrago-Pérez A,Saiz-Ladera C,Dueñas M,Paramio JM||Stem cell reviews (6:162)||2010|
Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology.
MA5-11344 was used in immunohistochemistry to study the role of embryonic stem cell transcription factor SOX2 in malonogenesis and Merkel cell ontogeny
|Laga AC,Lai CY,Zhan Q,Huang SJ,Velazquez EF,Yang Q,Hsu MY,Murphy GF||The American journal of pathology (176:903)||2010|
Involvement of the bulge region in primary scarring alopecia.
MA5-11344 was used in immunohistochemistry to study the role of the bulge region in primary scarring alopecia
|Pozdnyakova O,Mahalingam M||Journal of cutaneous pathology (35:922)||2008|
Elastic fiber staining and cytokeratin 15 expression pattern in trichoepithelioma and basal cell carcinoma.
MA5-11344 was used in immunohistochemistry to evaluate the diagnosis markers between trichoepithelioma and basal cell carcinoma
|Choi CW,Park HS,Kim YK,Lee SH,Cho KH||The Journal of dermatology (35:499)||2008|
Multipotent hair follicle stem cells promote repair of spinal cord injury and recovery of walking function.
MA5-11344 was used in immunohistochemistry to examine the application of hair follicle stem cells in spinal cord injury repair and recovery
|Amoh Y,Li L,Katsuoka K,Hoffman RM||Cell cycle (Georgetown, Tex.) (7:1865)||2008|
Hair cycle-dependent changes of alkaline phosphatase activity in the mesenchyme and epithelium in mouse vibrissal follicles.
MA5-11344 was used in immunohistochemistry to study alkaline phosphatase activity in mesenchyme and epithelium in mouse vibrissal follicles during the hair cycle
|Iida M,Ihara S,Matsuzaki T||Development, growth and differentiation (49:185)||2007|
Characterization of multipotent cells from human adult hair follicles.
MA5-11344 was used in immunohistochemistry and immunohistochemistry to characterize the multipotent endothelial cells from stripped human hair follicle
|Raposio E,Guida C,Baldelli I,Curto M,Fiocca R,Kunkl A,Robello G,Santi PL||Toxicology in vitro : an international journal published in association with BIBRA (21:320)||2007|
The effect of micropores in the surface of temperature-responsive culture inserts on the fabrication of transplantable canine oral mucosal epithelial cell sheets.
MA5-11344 was used in immunohistochemistry to study the use of micropores in the fabrication of transplantable canine oral mucosal epithelial cell sheets
|Murakami D,Yamato M,Nishida K,Ohki T,Takagi R,Yang J,Namiki H,Okano T||Biomaterials (27:5518)||2006|
Assessment of epidermal subpopulations and proliferation in healthy skin, symptomless and lesional skin of spreading psoriasis.
MA5-11344 was used in immunohistochemistry to study epidermal subpopulations and proliferation in healthy skin and psoriasis
|Körver JE,van Duijnhoven MW,Pasch MC,van Erp PE,van de Kerkhof PC||The British journal of dermatology (155:688)||2006|
Fabrication of transplantable human oral mucosal epithelial cell sheets using temperature-responsive culture inserts without feeder layer cells.
MA5-11344 was used in immunohistochemistry to develop a method for culturing transplantable human oral mucosal epithelial cell sheets without feeder layer cells
|Murakami D,Yamato M,Nishida K,Ohki T,Takagi R,Yang J,Namiki H,Okano T||Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs (9:185)||2006|
Effect of calcipotriol on epidermal cell populations in alefacept-treated psoriatic lesions.
MA5-11344 was used in immunohistochemistry to study the effect of treatment with calcipotriol and alefacept on epidermal cell populations in psoriatic lesions
|van Duijnhoven MW,Körver JE,Vissers WH,van Vlijmen-Willems IM,Pasch MC,van Erp PE,Van de Kerkhof PC||Journal of the European Academy of Dermatology and Venereology : JEADV (20:27)||2006|
Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves.
MA5-11344 was used in immunohistochemistry to investigate the transdifferentiation ability of hair follicle stem cells
|Amoh Y,Li L,Campillo R,Kawahara K,Katsuoka K,Penman S,Hoffman RM||Proceedings of the National Academy of Sciences of the United States of America (102:17734)||2005|
Multipotent nestin-positive, keratin-negative hair-follicle bulge stem cells can form neurons.
MA5-11344 was used in immunohistochemistry to study the differentiation of hair-follicle bulge stem cells
|Amoh Y,Li L,Katsuoka K,Penman S,Hoffman RM||Proceedings of the National Academy of Sciences of the United States of America (102:5530)||2005|
The combination of the Zenon labeling technique and microscopic image analysis to study cell populations in normal and psoriatic epidermis.
MA5-11344 was used in immunohistochemistry to study cell populations in normal and psoriatic epidermis using Zenon labelling and microscopic image analysis
|van Duijnhoven MW,van de Kerkhof PC,Pasch MC,Muys L,van Erp PE||Journal of cutaneous pathology (32:212)||2005|
Keratin immunohistochemistry in renal cell carcinoma subtypes and renal oncocytomas: a systematic analysis of 233 tumors.
MA5-11344 was used in immunohistochemistry to analyse keratin immunohistochemistry in renal cell carcinoma subtypes and renal oncocytomas
|Langner C,Wegscheider BJ,Ratschek M,Schips L,Zigeuner R||Virchows Archiv : an international journal of pathology (444:127)||2004|
Sub-cellular spectrochemical imaging of isolated human corneal cells employing synchrotron radiation-based Fourier-transform infrared microspectroscopy.
MA5-11344 was used in flow cytometry to investigate the use of SR-FTIR microspectroscopy to study human corneal cells at the subcellular level
|Fogarty SW,Patel II,Trevisan J,Nakamura T,Hirschmugl CJ,Fullwood NJ,Martin FL||The Analyst (138:240)||2013|
Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
MA5-11344 was used in flow cytometry to isolate and characterize stem cell-enriched human hair follicle bulge cells
|Ohyama M,Terunuma A,Tock CL,Radonovich MF,Pise-Masison CA,Hopping SB,Brady JN,Udey MC,Vogel JC||The Journal of clinical investigation (116:249)||2006|