|Tested species reactivity||Human, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Cytoskeletal preparation from rat colon.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:25-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 7 publications below|
MA1-06325 detects cytokeratin 17 in human and rat samples.
MA1-06325 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry.
The MA1-06325 immunogen is a cytoskeletal preparation from rat colon.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Impaired wound repair in adult endoglin heterozygous mice associated with lower NO bioavailability.
MA1-06325 was used in immunohistochemistry to study the role of reduced NO levels in the defective skin wound healing observed in adult endoglin (+/-) heterozygous mice
|Pérez-Gómez E,Jerkic M,Prieto M,del Castillo G,Martín-Villar E,Letarte M,Bernabeu C,Pérez-Barriocanal F,Quintanilla M,López-Novoa JM||The Journal of investigative dermatology (134:247)||2014|
CD200-expressing human basal cell carcinoma cells initiate tumor growth.
MA1-06325 was used in immunohistochemistry to study the abiliy of the CD200-positive human basal cell carcinoma cells to initiate tumor growth
|Colmont CS,Benketah A,Reed SH,Hawk NV,Telford WG,Ohyama M,Udey MC,Yee CL,Vogel JC,Patel GK||Proceedings of the National Academy of Sciences of the United States of America (110:1434)||2013|
Triple-negative breast cancer: immunohistochemical correlation with basaloid markers and prognostic value of survivin.
MA1-06325 was used in immunohistochemistry to study the prognostic value of survivin in patients with basaloid triplet-negative breast cancer
|Dogu GG,Ozkan M,Ozturk F,Dikilitas M,Er O,Ozturk A||Medical oncology (Northwood, London, England) (27:34)||2010|
Omics-based profiling of carcinoma of the breast and matched regional lymph node metastasis.
MA1-06325 was used in immunohistochemistry to investigate the relationship between axillary lymph node metastases and primary breast tumors
|Li J,Gromov P,Gromova I,Moreira JM,Timmermans-Wielenga V,Rank F,Wang K,Li S,Li H,Wiuf C,Yang H,Zhang X,Bolund L,Celis JE||Proteomics (8:5038)||2008|
Keratin profiling in the developing human prostate. A different approach to understanding epithelial lineage.
MA1-06325 was used in immunohistochemistry to investigate the distribution of keratin in prostate during development
|Trompetter M,Smedts F,van der Wijk J,Schoots C,de Jong HJ,Hopman A,de la Rosette J||Anticancer research (28:237)||2008|
Basoluminal carcinoma: a new biologically and prognostically distinct entity between basal and luminal breast cancer.
MA1-06325 was used in immunohistochemistry to study the characteristics of basoluminal carcinoma
|Laakso M,Tanner M,Nilsson J,Wiklund T,Erikstein B,Kellokumpu-Lehtinen P,Malmström P,Wilking N,Bergh J,Isola J||Clinical cancer research : an official journal of the American Association for Cancer Research (12:4185)||2006|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA1-06325 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|