Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), hepatocarcinoma (B), stomach cancer (C) and colon cancer tissue (D), showing cytoplasmic location using Cytokeratin 18 monoclonal antibody (Product # MA5-15458) followed with DAB staining
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15458 targets Cytokeratin 18 in IHC and WB applications and shows reactivity with Human samples.
The MA5-15458 immunogen is purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
MA5-15458 detects Cytokeratin 18 which has a predicted molecular weight of approximately 48kDa.
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.