Proteintech

Cytokeratin 18 Polyclonal Antibody

Product Image Gallery

Cytokeratin 18 Antibody in Immunofluorescence (IF) — IF result of CK18 antibody (10830-1-AP, 1:50 ) with 1% PLP fixed the APical sustentacular cells of adult mouse olfactory epithelium. (Green: CK18; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.

Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Cytokeratin 18 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytokeratin 18 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug Cytokeratin 18 antibody (10830-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Cytokeratin 18 Antibody in Immunoprecipitation (IP) — IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1000

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:1000-1:4000

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cytokeratin 18 Fusion Protein Ag1260

Conjugate

Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; Endo B; K 18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; Keratin D; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; type I cytoskeletal 18

Gene Aliases: CK-18; CK18; CYK18; K18; Kerd; Krt1-18; KRT18; PIG46

UniProt ID: (Human) P05783, (Mouse) P05784

Entrez Gene ID: (Human) 3875, (Mouse) 16668

Molecular Function: cytoskeletal protein intermediate filament structural protein