Proteintech

Cytokeratin 18 Polyclonal Antibody

Product Image Gallery

Cytokeratin 18 Antibody in Immunocytochemistry (ICC/IF) — IF result of CK18 antibody (10830-1-AP, 1:50 ) with 1% PLP fixed the APical sustentacular cells of adult mouse olfactory epithelium. (Green: CK18; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.

Cytokeratin 18 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using 10830-1-AP (Cytokeratin 18 antibody at dilution of 1:1000 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Cytokeratin 18 Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 10830-1-AP (Cytokeratin 18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytokeratin 18 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug Cytokeratin 18 antibody (10830-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Cytokeratin 18 Antibody in Immunoprecipitation (IP) — IP result of anti-Cytokeratin 18 (IP:10830-1-AP, 3ug; Detection:10830-1-AP 1:2000) with A431 cells lysate 3300ug.

Product Details

10830-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 16 publications 16 publications
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 7 publications 7 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytokeratin 18 Fusion Protein Ag1260 (127-430 aa encoded by BC000180) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.19 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: SHYFKIIED LRAQIFANTV DNARIVLQID NARLAADDFR VKYETELAMR QSVENDIHGL RKVIDDTNIT RLQLETEIEA LKEELLFMKK NHEEEVKGLQ AQIASSGLTV EVDAPKSQDL AKIMADIRAQ YDELARKNRE ELDKYWSQQI EESTTVVTTQ SAEVGAAETT LTELRRTVQS LEIDLDSMRN LKASLENSLR EVEARYALQM EQLNGILLHL ESELAQTRAE GQRQAQEYEA LLNIKVKLEA EIATYRRLLE DGEDFNLGDA LDSSNSMQTI QKTTTRRIVD GKVVSETNDT KVLRH (127-430 aa encoded by BC000180)

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; Endo B; K 18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; Keratin D; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; type I cytoskeletal 18

Gene Aliases: CK-18; CK18; CYK18; K18; Kerd; Krt1-18; KRT18; PIG46

UniProt ID: (Human) P05783, (Mouse) P05784

Entrez Gene ID: (Human) 3875, (Mouse) 16668

Function(s)

cell cycle anatomical structure morphogenesis viral process tumor necrosis factor-mediated signaling pathway Golgi to plasma membrane CFTR protein transport negative regulation of apoptotic process intermediate filament cytoskeleton organization extrinsic apoptotic signaling pathway hepatocyte apoptotic process cell-cell adhesion

Process(es)

structural molecule activity protein binding poly(A) RNA binding scaffold protein binding cadherin binding involved in cell-cell adhesion