Immunofluorescent analysis of Cytokeratin 18 showing staining in the cytoskeleton of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a Cytokeratin 18 polyclonal antibody (Product # PA5-28279) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 73 and 300 of Human Cytokeratin 18|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28279 targets Cytokeratin 18 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28279 immunogen is recombinant fragment corresponding to a region within amino acids 73 and 300 of Human Cytokeratin 18.
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.