|Tested species reactivity||Dog, Chicken, Hamster, Human, Mouse, Pig, Rabbit, Rat, Zebrafish|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeletal preparation from cells.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-06326 detects cytokeratin 18 in human, mouse, rat, hamster, rabbit, chicken, canine, zebrafish and swine samples.
MA1-06326 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry, and immunohistochemistry.
The MA1-06326 immunogen is a cytoskeletal preparation from cells.
Store at 4°C or in small aliquots at -20°C.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Polyethylene glycol (PEG)-induced mouse model of choroidal neovascularization.
MA1-06326 was used in immunohistochemistry to evaluate a murine wet age-related macular degeneration model for the induction of choroidal neovascularization
|Lyzogubov VV,Tytarenko RG,Liu J,Bora NS,Bora PS||The Journal of biological chemistry (286:16229)||2011|