Immunofluorescent analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 (A53-B/A2.26 (Ks19.1) ) Mouse Monoclonal Antibody (MA5-12663) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Sheep, Human, Mouse, Not Applicable, Rhesus monkey|
|Host / Isotype||Mouse / IgG2a, lambda|
|Immunogen||Human breast cancer MCF-7 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12663 targets Cytokeratin 19 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The MA5-12663 immunogen is human breast cancer MCF-7 cells.
Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thyroid Paraganglioma Diagnosed by Fine-Needle Aspiration Biopsy, Correlated With Histopathological Findings: Report of a Case.
MA5-12663 was used in immunohistochemistry to characterize a case where diagnosis of thyroid paraganglioma occurred by fine-needle aspiration biopsy and histopathological findings
|Çetin ¿,Kir G,Yilmaz M||Diagnostic cytopathology (44:643)||2016|
Diagnostic criteria of well differentiated thyroid tumor of uncertain malignant potential; a histomorphological and immunohistochemical appraisal.
MA5-12663 was used in immunohistochemistry to assess the properties of well differentiated thyroid tumors by histomorphological and immunohistochemical analysis
|Yassin Fel-Z||Journal of the Egyptian National Cancer Institute (27:59)||2015|
Keratinocyte growth factor-2 and autologous serum potentiate the regenerative effect of mesenchymal stem cells in cornea damage in rats.
MA5-12663 was used in immunohistochemistry to study the use of mesenchymal stem cells to repair rat corneal damage and the additional therapeutic effect obtained from the use of KGF-2 and autologous serum
|P¿narl¿ FA,Okten G,Beden U,F¿¿g¿n T,Kefeli M,Kara N,Duru F,Tomak L||International journal of ophthalmology (7:211)||2014|
A renewable tissue resource of phenotypically stable, biologically and ethnically diverse, patient-derived human breast cancer xenograft models.
MA5-12663 was used in immunohistochemistry to establish a panel of biologically diverse and phenotypically stable transplantable cell lines from patient tissues for use in xenograft models of breast cancer
|Zhang X,Claerhout S,Prat A,Dobrolecki LE,Petrovic I,Lai Q,Landis MD,Wiechmann L,Schiff R,Giuliano M,Wong H,Fuqua SW,Contreras A,Gutierrez C,Huang J,Mao S,Pavlick AC,Froehlich AM,Wu MF,Tsimelzon A,Hilsenbeck SG,Chen ES,Zuloaga P,Shaw CA,Rimawi MF,Perou CM,Mills GB,Chang JC,Lewis MT||Cancer research (73:4885)||2013|
|Not Applicable||Not Cited||
Gremlin in the pathogenesis of hepatocellular carcinoma complicating chronic hepatitis C: an immunohistochemical and PCR study of human liver biopsies.
MA5-12663 was used in immunohistochemistry to determine the role of secretory products of fibrous tissue in the development of hepatocellular carcinoma
|Guimei M,Baddour N,Elkaffash D,Abdou L,Taher Y||BMC research notes (5:null)||2012|
Primary pure squamous cell carcinoma of the thyroid: report and histogenic consideration of a case involving a BRAF mutation.
MA5-12663 was used in immunohistochemistry to study a case of primary squamous cell carcinoma of the thyroid
|Ko YS,Hwang TS,Han HS,Lim SD,Kim WS,Oh SY||Pathology international (62:43)||2012|
Mucinous tubular and spindle cell carcinoma of kidney and problems in diagnosis.
MA5-12663 was used in immunohistochemistry to study problems in diagnosing mucinous tubular and spindle cell carcinoma of kidney
|Sarsik B,S¿m¿¿r A,Karaarslan S,Sen S||Turk patoloji dergisi (27:116)||2011|
Tumor-in-tumor of the thyroid with basaloid differentiation: a lesion with a solid cell nest neoplastic component?
MA5-12663 was used in immunohistochemistry to report on a case of tumor-in-tumor of the thyroid with basaloid differentiation
|Eloy C,Vinagre J,Cameselle-Teijeiro J,Paiva ME,Soares P,Sobrinho-Simões M||International journal of surgical pathology (19:276)||2011|
Tissue proteomics of the human mammary gland: towards an abridged definition of the molecular phenotypes underlying epithelial normalcy.
MA5-12663 was used in immunohistochemistry to conduct proteomic profiling of human mammary glands
|Moreira JM,Cabezón T,Gromova I,Gromov P,Timmermans-Wielenga V,Machado I,Llombart-Bosch A,Kroman N,Rank F,Celis JE||Molecular oncology (4:539)||2010|
In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands.
MA5-12663 was used in immunohistochemistry to investigate the integration of sweat glands into engineered skin constructs in vitro and in vivo in order to improve the quality of tissue regeneration
|Huang S,Xu Y,Wu C,Sha D,Fu X||Biomaterials (31:5520)||2010|
Absence of the BRAF mutation in HBME1+ and CK19+ atypical cell clusters in Hashimoto thyroiditis: supportive evidence against preneoplastic change.
MA5-12663 was used in immunohistochemistry to study whether HBMF1+/CD19+ clusters in Hashimoto's thyroiditis are potential precursors of papilliary thyroid carcinoma
|Nasr MR,Mukhopadhyay S,Zhang S,Katzenstein AL||American journal of clinical pathology (132:906)||2009|
Antigen expression of human eccrine sweat glands.
MA5-12663 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands
|Li HH,Zhou G,Fu XB,Zhang L||Journal of cutaneous pathology (36:318)||2009|
Expression of estrogen-metabolizing enzymes and estrogen receptors in cholelithiasis gallbladder.
MA5-12663 was used in immunohistochemistry to investigate the distribution of estrogen-metabolizing enzymes and estrogen receptors in gallbladder specimens from patients with cholelithiasis
|Svoboda M,Sellner F,Ekmekcioglu C,Klimpfinger M,Jaeger W,Thalhammer T||Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie (62:690)||2008|
15-prostaglandin dehydrogenase expression alone or in combination with ACSM1 defines a subgroup of the apocrine molecular subtype of breast carcinoma.
MA5-12663 was used in immunohistochemistry to examine the diagnostic significance of 15-prostaglandin dehydrogenase and ACSM1 in breast carcinoma
|Celis JE,Gromov P,Cabezón T,Moreira JM,Friis E,Jirström K,Llombart-Bosch A,Timmermans-Wielenga V,Rank F,Gromova I||Molecular and cellular proteomics : MCP (7:1795)||2008|
Mammary gland development in early pubertal female macaques.
MA5-12663 was used in immunohistochemistry to investigate the effect of estradiol on early ductal and lobular development
|Wood CE,Hester JM,Cline JM||Toxicologic pathology (35:795)||2007|
An immunohistochemical profile of giant cell carcinoma of the larynx.
MA5-12663 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx
|Gurbuz Y,Kose N,Aydin O,Ozturk M||Auris, nasus, larynx (34:413)||2007|
Immunohistochemical markers in diagnosis of papillary thyroid carcinoma: Utility of HBME1 combined with CK19 immunostaining.
MA5-12663 was used in immunohistochemistry to study the utility of HBME1 and CK19 as diagnostic immunohistochemical markers of papillary thyroid carcinoma
|Nasr MR,Mukhopadhyay S,Zhang S,Katzenstein AL||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (19:1631)||2006|
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
MA5-12663 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver
|Yesim G,Gupse T,Zafer U,Ahmet A||Journal of hepato-biliary-pancreatic surgery (12:502)||2005|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA5-12663 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Galectin-3 and HBME-1 improve the accuracy of core biopsy in indeterminate thyroid nodules.
MA5-12663 was used in immunohistochemistry - paraffin section to investigate using additional markers for core needle biopsy of thyroid nodules.
|Trimboli P,Guidobaldi L,Amendola S,Nasrollah N,Romanelli F,Attanasio D,Ramacciato G,Saggiorato E,Valabrega S,Crescenzi A||Endocrine (52:39)||2016|
Keratin 19 protein expression is an independent predictor of survival in human hepatocellular carcinoma.
MA5-12663 was used in immunohistochemistry - paraffin section to study the prognostic effect of Keratin 19 expression on survival of patients with human hepatocellular carcinoma
|Fatourou E,Koskinas J,Karandrea D,Palaiologou M,Syminelaki T,Karanikolas M,Felekouras E,Antoniou E,Manesis EK,Delladetsima J,Tiniakos D||European journal of gastroenterology and hepatology (27:1094)||2015|
|Not Applicable||Not Cited||
Potential Cross-Talk between Alternative and Classical NF-¿B Pathways in Prostate Cancer Tissues as Measured by a Multi-Staining Immunofluorescence Co-Localization Assay.
MA5-12663 was used in immunohistochemistry - paraffin section to research by use of a multi-staining immunofluorescence co-localization assay to study potential cross-talk beteween classical and alternative NF-kappaB pathways in prostate cancer tissues
|Labouba I,Le Page C,Communal L,Kristessen T,You X,Péant B,Barrès V,Gannon PO,Mes-Masson AM,Saad F||PloS one (10:null)||2015|
|Not Applicable||Not Cited||
High mammographic density is associated with an increase in stromal collagen and immune cells within the mammary epithelium.
MA5-12663 was used in immunohistochemistry - paraffin section to study the increase in stromal collagen and immune cells within the mammary epithelium shown as high mammographic density
|Huo CW,Chew G,Hill P,Huang D,Ingman W,Hodson L,Brown KA,Magenau A,Allam AH,McGhee E,Timpson P,Henderson MA,Thompson EW,Britt K||Breast cancer research : BCR (17:null)||2015|
Clinicopathological characteristics of kidney mucinous tubular and spindle cell carcinoma.
MA5-12663 was used in immunohistochemistry - paraffin section to describe the clinicopathologically features of two cases of kidney mucinous tubular and spindle cell carcinoma
|Chen Q,Gu Y,Liu B||International journal of clinical and experimental pathology (8:1007)||2015|
Modelling breast cancer requires identification and correction of a critical cell lineage-dependent transduction bias.
MA5-12663 was used in immunocytochemistry to study and identify critical cell lineage-dependent transduction bias to model breast cancer properly
|Hines WC,Yaswen P,Bissell MJ||Nature communications (6:null)||2015|
Establishment and characterization of a telomerase immortalized human gingival epithelial cell line.
MA5-12663 was used in immunocytochemistry to develop a telomerase-imortalized human primary gingival epithelial cell line
|Moffatt-Jauregui CE,Robinson B,de Moya AV,Brockman RD,Roman AV,Cash MN,Culp DJ,Lamont RJ||Journal of periodontal research (48:713)||2013|
Comparison of a poly-L-lactide-co-¿-caprolactone and human amniotic membrane for urothelium tissue engineering applications.
MA5-12663 was used in immunocytochemistry to study the potential of poly-L-lactide-co-epsilon-caprolactone and human amniotic membrane in urothelial tissue engineering applications
|Sartoneva R,Haimi S,Miettinen S,Mannerström B,Haaparanta AM,Sándor GK,Kellomäki M,Suuronen R,Lahdes-Vasama T||Journal of the Royal Society, Interface (8:671)||2011|
An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma in situ.
MA5-12663 was used in immunocytochemistry to develop an in vivo model for noninvasive breast tumors
|Behbod F,Kittrell FS,LaMarca H,Edwards D,Kerbawy S,Heestand JC,Young E,Mukhopadhyay P,Yeh HW,Allred DC,Hu M,Polyak K,Rosen JM,Medina D||Breast cancer research : BCR (11:null)||2009|
Nucleostemin as a possible progenitor marker of corneal epithelial cells.
MA5-12663 was used in immunocytochemistry to evaluate nucleostemin as a corneal epithelial stem cell marker
|Kawashima M,Kawakita T,Yoshida S,Shimmura S,Tsubota K||Molecular vision (15:1162)||2009|
Isolation and identification of stem cells from adult cashmere goat skin.
MA5-12663 was used in immunocytochemistry to examine the totipotency of adult cashmere goat skin epithelia
|Liu Y,Zhou H,Gao F||International journal of dermatology (47:551)||2008|
Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus.
MA5-12663 was used in immunocytochemistry to characterize the putative epidermal stem cells from Cashmere goat fetus
|Islam MS,Zhou H||European journal of dermatology : EJD (17:302)||2007|
Isolation, immortalization, and characterization of a human breast epithelial cell line with stem cell properties.
MA5-12663 was used in immunocytochemistry and immunohistochemistry to isolate and characterize a human breast epithelial cell line with stem cell properties
|Gudjonsson T,Villadsen R,Nielsen HL,Rønnov-Jessen L,Bissell MJ,Petersen OW||Genes and development (16:693)||2002|
Establishment and characterization of novel cell lines from sinonasal undifferentiated carcinoma.
MA5-12663 was used in western blot to characterise novel sinonasal undifferentiated carcinoma cell lines
|Takahashi Y,Kupferman ME,Bell D,Jiffar T,Lee JG,Xie TX,Li NW,Zhao M,Frederick MJ,Gelbard A,Myers JN,Hanna EY||Clinical cancer research : an official journal of the American Association for Cancer Research (18:6178)||2012|
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
MA5-12663 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
|Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS||Research in veterinary science (88:285)||2010|
Down-regulation of the tumor suppressor protein 14-3-3sigma is a sporadic event in cancer of the breast.
MA5-12663 was used in western blot to study the role of the tumor suppressor protein 14-3-3sigma in breask cancer
|Moreira JM,Ohlsson G,Rank FE,Celis JE||Molecular and cellular proteomics : MCP (4:555)||2005|