Immunofluorescent analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 (BA17) Mouse Monoclonal Antibody (MA5-12319) at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Detergent soluble extract of human mammary epithelium|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12319 targets Cytokeratin 19 in IHC (P) and WB applications and shows reactivity with Human samples.
The MA5-12319 immunogen is detergent soluble extract of human mammary epithelium.
Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different humantissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Histochemical and immunohistochemical characterization of chordoma in ferrets.
MA5-12319 was used in immunohistochemistry - paraffin section to use of ferrets to characterize immunohistochemical and histochemical levels of chordoma
|Yui T,Ohmachi T,Matsuda K,Okamoto M,Taniyama H||The Journal of veterinary medical science (77:467)||2015|
Anti-fibrotic effects of fresh and cryopreserved human amniotic membrane in a rat liver fibrosis model.
MA5-12319 was used in immunohistochemistry to compare the anti-fibrotic activity fresh and cryopreserved human amniotic membranes
|Ricci E,Vanosi G,Lindenmair A,Hennerbichler S,Peterbauer-Scherb A,Wolbank S,Cargnoni A,Signoroni PB,Campagnol M,Gabriel C,Redl H,Parolini O||Cell and tissue banking (14:475)||2013|
Ocular melanoma and mammary mucinous carcinoma in an African lion.
MA5-12319 was used in immunohistochemistry to report on a case of ocular melanoma and mammary mucinous carcinoma in an African lion
|Cagnini DQ,Salgado BS,Linardi JL,Grandi F,Rocha RM,Rocha NS,Teixeira CR,Del Piero F,Sequeira JL||BMC veterinary research (8:null)||2012|
Universal markers of thyroid malignancies: galectin-3, HBME-1, and cytokeratin-19.
MA5-12319 was used in immunohistochemistry to detect malignancies using immunohistochemical markers
|Barut F,Onak Kandemir N,Bektas S,Bahadir B,Keser S,Ozdamar SO||Endocrine pathology (21:80)||2010|
An integrative computational model for intestinal tissue renewal.
MA5-12319 was used in immunohistochemistry to evaluate a computational model for intestinal epithelial cell renewal
|van Leeuwen IM,Mirams GR,Walter A,Fletcher A,Murray P,Osborne J,Varma S,Young SJ,Cooper J,Doyle B,Pitt-Francis J,Momtahan L,Pathmanathan P,Whiteley JP,Chapman SJ,Gavaghan DJ,Jensen OE,King JR,Maini PK,Waters SL,Byrne HM||Cell proliferation (42:617)||2009|
Transplantation of allogeneic and xenogeneic placenta-derived cells reduces bleomycin-induced lung fibrosis.
MA5-12319 was used in immunohistochemistry to investigate the usefulness of placenta-derived cell transplantation on bleomycin-mediated lung fibrosis
|Cargnoni A,Gibelli L,Tosini A,Signoroni PB,Nassuato C,Arienti D,Lombardi G,Albertini A,Wengler GS,Parolini O||Cell transplantation (18:405)||2009|
Modulation by retinoic acid (RA) of squamous cell differentiation, cellular RA-binding proteins, and nuclear RA receptors in human head and neck squamous cell carcinoma cell lines.
MA5-12319 was used in immunohistochemistry to investigate the effect of retinoic acid on squamous cell differentiation.
|Zou CP,Clifford JL,Xu XC,Sacks PG,Chambon P,Hong WK,Lotan R||Cancer research (54:5479)||1994|
Characterizing and optimizing poly-L-lactide-co-¿-caprolactone membranes for urothelial tissue engineering.
MA5-12319 was used in immunocytochemistry to develop poly-l-lactide-co-epsilon-caprolactone-based membranes for tissue engineering of human urothelial cells
|Sartoneva R,Haaparanta AM,Lahdes-Vasama T,Mannerström B,Kellomäki M,Salomäki M,Sándor G,Seppänen R,Miettinen S,Haimi S||Journal of the Royal Society, Interface (9:3444)||2012|