Proteintech

Cytokeratin 19 Polyclonal Antibody

Product Image Gallery

Cytokeratin 19 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Cytokeratin 19 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10712-1-AP (Cytokeratin 19 antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Cytokeratin 19 Antibody in Western Blot (WB) — Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10712-1-AP (KRT19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytokeratin 19 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug Cytokeratin 19 antibody (10712-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

10712-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 22 publications 22 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000-1:6,400	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 7 publications 7 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytokeratin 19 Fusion Protein Ag1085 (80-400 aa encoded by BC007628) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: EKLTMQNLN DRLASYLDKV RALEAANGEL EVKIRDWYQK QGPGPSRDYS HYYTTIQDLR DKILGATIEN SRIVLQIDNA RLAADDFRTK FETEQALRMS VEADINGLRR VLDELTLART DLEMQIEGLK EELAYLKKNH EEEISTLRGQ VGGQVSVEVD SAPGTDLAKI LSDMRSQYEV MAEQNRKDAE AWFTSRTEEL NREVAGHTEQ LQMSRSEVTD LRRTLQGLEI ELQSQLSMKA ALEDTLAETE ARFGAQLAHI QALISGIEAQ LGDVRADSER QNQEYQRLMD IKSRLEQEIA TYRSLLEGQE DHYNNLSASK VL (80-400 aa encoded by BC007628)

Target Information

Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Bioinformatics

Protein Aliases: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; Cytokeratin-19; K19; keratin 19, type I; keratin complex 1, acidic, gene 19; Keratin, type I cytoskeletal 19; keratin, type I, 40-kd; Keratin-19; MGC15366

Gene Aliases: AI663979; CK19; EndoC; K19; K1CS; Krt-1.19; Krt1-19; KRT19

UniProt ID: (Human) P08727, (Mouse) P19001

Entrez Gene ID: (Human) 3880, (Mouse) 16669

Function(s)

Notch signaling pathway viral process response to estrogen sarcomere organization cell differentiation involved in embryonic placenta development

Process(es)

structural constituent of cytoskeleton protein binding structural constituent of muscle protein complex binding structural molecule activity