|Tested species reactivity||Human, Zebrafish|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeletal preparation of human bladder carcinoma cell line T24.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06329 detects cytokeratin 19 in human and zebrafish samples.
MA1-06329 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry and immunohistochemistry.
The MA1-06329 immunogen is a cytoskeletal preparation of human bladder carcinoma cell line T24.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis.
MA1-06329 was used in immunohistochemistry to establish an in vitro system to study three-dimensional corneal differentiation
|Ouyang H,Xue Y,Lin Y,Zhang X,Xi L,Patel S,Cai H,Luo J,Zhang M,Zhang M,Yang Y,Li G,Li H,Jiang W,Yeh E,Lin J,Pei M,Zhu J,Cao G,Zhang L,Yu B,Chen S,Fu XD,Liu Y,Zhang K||Nature (511:358)||2014|
High expression of Galectin-1 in pancreatic stellate cells plays a role in the development and maintenance of an immunosuppressive microenvironment in pancreatic cancer.
MA1-06329 was used in immunohistochemistry to investigate the role of galectin-1 in the immunosuppressive microenvironment in pancreatic tumors
|Tang D,Yuan Z,Xue X,Lu Z,Zhang Y,Wang H,Chen M,An Y,Wei J,Zhu Y,Miao Y,Jiang K||International journal of cancer (130:2337)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA1-06329 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Dormant but migratory tumour cells in desmoplastic stroma of invasive ductal carcinomas.
MA1-06329 was used in immunohistochemistry to characterize solitary primary tumor cells around the breast cancer perimeter
|Raviraj V,Zhang H,Chien HY,Cole L,Thompson EW,Soon L||Clinical and experimental metastasis (29:273)||2012|
A novel mechanism of keratin cytoskeleton organization through casein kinase I¿ and FAM83H in colorectal cancer.
MA1-06329 was used in immunocytochemistry and western blot to study the role of casein kinase I-alpha and FAM88H in the keratin cytoskeletal rearrangements observed in colorectal cancer
|Kuga T,Kume H,Kawasaki N,Sato M,Adachi J,Shiromizu T,Hoshino I,Nishimori T,Matsubara H,Tomonaga T||Journal of cell science (126:4721)||2013|
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
MA1-06329 was used in immunocytochemistry to investigate the transdifferentiation of human embryonic pancreatic cells to hepatocytes
|Sumitran-Holgersson S,Nowak G,Thowfeequ S,Begum S,Joshi M,Jaksch M,Kjaeldgaard A,Jorns C,Ericzon BG,Tosh D||Cell transplantation (18:183)||2009|