Immunofluorescent analysis of Cytokeratin 31 showing staining in the cytoplasm and nucleus of MCF-7 cells. MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Cytokeratin 31 polyclonal antibody (Product # PA5-29522) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 175 and 416 of Human Cytokeratin 31|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29522 targets Cytokeratin 31 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-29522 immunogen is recombinant fragment corresponding to a region within amino acids 175 and 416 of Human Cytokeratin 31.
The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.
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