Immunofluorescent analysis of Cytokeratin 4 showing staining in the cytoskeleton of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a Cytokeratin 4 polyclonal antibody (Product # PA5-29463) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 317 and 505 of Human Cytokeratin 4|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29463 targets Cytokeratin 4 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29463 immunogen is recombinant protein fragment corresponding to a region within amino acids 317 and 505 of Human Cytokeratin 4.
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.