|Tested species reactivity||Dog, Cat, Human, Zebrafish|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratin preparation extracted from human esophagus.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
MA1-06313 detects cytokeratin 4 in human, canine, zebrafish, and feline samples.
MA1-06313 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry, and immunoprecipitation.
The MA1-06313 immunogen is a cytokeratin preparation extracted from human esophagus.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
MA1-06313 was used in immunohistochemistry to study the expression pattern of stem cells in the bulbar conjunctival epithelium
|Qi H,Zheng X,Yuan X,Pflugfelder SC,Li DQ||Journal of cellular physiology (225:180)||2010|
Modulation of gingival epithelial phenotypes by interactions with regionally defined populations of fibroblasts.
MA1-06313 was used in immunohistochemistry to study the role of interactions with regionally defined fibroblast populations on the phenotypes of gingival epithelial cells using an organotypic model
|Locke M,Hyland PL,Irwin CR,Mackenzie IC||Journal of periodontal research (43:279)||2008|