Proteintech

Cytokeratin 6A Polyclonal Antibody

Product Image Gallery

Cytokeratin 6A Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of A431 cells using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Cytokeratin 6A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 10590-1-AP (Cytokeratin 6A antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Cytokeratin 6A Antibody in Western Blot (WB) — Rat skin tissue were subjected to SDS PAGE followed by western blot with 10590-1-AP (Cytokeratin 6A antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Cytokeratin 6A Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 6A antibody (10590-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Western Blot (WB)

1:10,000-1:50,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cytokeratin 6A Fusion Protein Ag0882

Conjugate

Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 60-kDa keratin; CK-6A; CK-6D; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Hom s 5; K6A; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 6A, , type II; keratin 6A, type II; keratin complex 2, basic, gene 6a; keratin complex 2, basic, gene 6c; keratin complex 2, gene 6a; keratin K6h; keratin, epidermal type II, K6A; Keratin, type II cytoskeletal 6A; Keratin-6-alpha; Keratin-6A; keratin-6C; mK6-alpha; type-II keratin Kb12; Type-II keratin Kb6

Gene Aliases: 2310016L08Rik; CK-6C; CK-6E; CK6A; CK6C; CK6D; K6A; K6C; K6D; Ker2; Krt2-6; Krt2-6a; Krt2-6c; Krt6; KRT6A; KRT6C; KRT6D; mK6[a]; MK6a; PC3

UniProt ID: (Human) P02538, (Mouse) P50446

Entrez Gene ID: (Human) 3853, (Mouse) 16687

Molecular Function: cytoskeletal protein intermediate filament structural protein