Immunofluorescence analysis of Cytokeratin 7 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 7 (OV-TL 12/30) Mouse Monoclonal Antibody (Product # MA5-11986) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Fish, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||OTN II ovarian carcinoma cell line|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11986 targets Cytokeratin 7 in IHC (P) applications and shows reactivity with Human samples.
The MA5-11986 immunogen is oTN II ovarian carcinoma cell line.
Cytokeratin 7 is a basic cytokeratin which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
MA5-11986 was used in immunohistochemistry to study a northern New England hospital and increased utilization, verification, and clinical implications of immunocytochemistry
|Sauter JL,Ambaye AB,Mount SL||Diagnostic cytopathology (43:688)||2015|
New diagnostic markers in salivary gland tumors.
MA5-11986 was used in immunohistochemistry to study the expresion of tumor antigens in order to identify novel diagnostic markers of parotid gland tumors
|Schneider S,Kloimstein P,Pammer J,Brannath W,Grasl MCh,Erovic BM||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (271:1999)||2014|
Amnion-derived mesenchymal stromal cells show a mesenchymal-epithelial phenotype in culture.
MA5-11986 was used in immunohistochemistry to study the mesenchymal-epithelial phenotype displayed by cultured mesenchymal stromal cells derived from the amniotic membrane
|König J,Lang I,Siwetz M,Fröhlich J,Huppertz B||Cell and tissue banking (15:193)||2014|
Tumor-induced STAT3 activation in monocytic myeloid-derived suppressor cells enhances stemness and mesenchymal properties in human pancreatic cancer.
MA5-11986 was used in immunohistochemistry to study the mechanism by which STAT3 activation promotes stemness in pancreatic cancer cancer stem cells
|Panni RZ,Sanford DE,Belt BA,Mitchem JB,Worley LA,Goetz BD,Mukherjee P,Wang-Gillam A,Link DC,Denardo DG,Goedegebuure SP,Linehan DC||Cancer immunology, immunotherapy : CII (63:513)||2014|
Three-dimensional culture and identification of human eccrine sweat glands in matrigel basement membrane matrix.
MA5-11986 was used in immunohistochemistry to develop a matrigel-based system for the 3-dimensional culture of human eccrine sweat glands
|Li H,Chen L,Zhang M,Tang S,Fu X||Cell and tissue research (354:897)||2013|
Identification of novel target proteins in sebaceous gland carcinoma.
MA5-11986 was used in immunohistochemistry to identify novel therapeutic target proteins in sebaceous gland carcinoma
|Erovic BM,Al Habeeb A,Harris L,Goldstein DP,Kim D,Ghazarian D,Irish JC||Head and neck (35:642)||2013|
Specimen quality evaluation in Canadian biobanks participating in the COEUR repository.
MA5-11986 was used in immunohistochemistry to study the quality of biological samples held in Canadian biobanks forming part of the COEUR repository
|Le Page C,Köbel M,de Ladurantaye M,Rahimi K,Madore J,Babinszky S,Bachvarov DR,Bachvarova M,Beauchamp MC,Cass CE,Chadwick D,Colleen C,Damaraju S,Dufour J,Gotlieb WH,Kalloger SE,Portelance L,McAlpine JN,Matte I,Piché A,Shaw P,Roehrl MH,Vanderhyden BC,Watson PH,Huntsman DG,Provencher DM,Mes-Masson AM||Biopreservation and biobanking (11:83)||2013|
Serrated pathway adenocarcinomas: molecular and immunohistochemical insights into their recognition.
MA5-11986 was used in immunohistochemistry to establish criteria for the recognition of serrated pathway adenocarcinomas
|Gurzu S,Szentirmay Z,Toth E,Bara T,Bara T,Jung I||PloS one (8:null)||2013|
Adenocarcinoma with sarcomatous dedifferentiation arising from mature cystic teratoma of the anterior mediastinum.
MA5-11986 was used in immunohistochemistry to report on a case of adenocarcinoma with sarcomatous dedifferentiation arising from mature cystic teratoma of the anterior mediastinum
|Kim JY,Lee CH,Park WY,Kim JY,Kim AR,Shin N,Park DY,Huh GY||Pathology, research and practice (208:741)||2012|
Biomarkers of parathyroid carcinoma.
MA5-11986 was used in immunohistochemistry to establish a biomarker panel suitable for the identification of parathyroid carcinomas
|Erovic BM,Harris L,Jamali M,Goldstein DP,Irish JC,Asa SL,Mete O||Endocrine pathology (23:221)||2012|
Miliary brain metastases from occult lung adenocarcinoma: Radiologic and histopathologic confirmation.
MA5-11986 was used in immunohistochemistry to report on a case of miliary brain metastases from occult lung adenocarcinoma
|Kahveci R,Gürer B,Kaygusuz G,Sekerci Z||Journal of neurosciences in rural practice (3:386)||2012|
Significance of TRIM29 and ß-catenin expression in non-small-cell lung cancer.
MA5-11986 was used in immunohistochemistry to study non-small cell lung cancer and the potential clinicopathological significance of TRIM29 and beta-catenin expression
|Zhou ZY,Yang GY,Zhou J,Yu MH||Journal of the Chinese Medical Association : JCMA (75:269)||2012|
A new case of primary signet-ring cell carcinoma of the cervix with prominent endometrial and myometrial involvement: Immunohistochemical and molecular studies and review of the literature.
MA5-11986 was used in immunohistochemistry to report on a case of primary signet-ring cell carcinoma of the cervix with endometrial and myometrial involvement
|Giordano G,Pizzi S,Berretta R,D'Adda T||World journal of surgical oncology (10:null)||2012|
Aberrant pattern of the cytokeratin 7/cytokeratin 20 immunophenotype in colorectal adenocarcinomas with BRAF mutations.
MA5-11986 was used in immunohistochemistry to study the aberrant cytokeratin 7/cytokeratin 20 immunophenotype in colorectal adenocarcinomas with BRAF mutations
|Gurzu S,Jung I||Pathology, research and practice (208:163)||2012|
The value of CDX2 and cytokeratins 7 and 20 expression in differentiating colorectal adenocarcinomas from extraintestinal gastrointestinal adenocarcinomas: cytokeratin 7-/20+ phenotype is more specific than CDX2 antibody.
MA5-11986 was used in immunohistochemistry to study the value of CDX2 and cytokeratin 7 and 20 expression in differentiating colorectal adenocarcinomas from extraintestinal gastrointestinal adenocarcinomas
|Bayrak R,Haltas H,Yenidunya S||Diagnostic pathology (7:null)||2012|
Two novel mutations in African and Asian children with progressive familial intrahepatic cholestasis type 3.
MA5-11986 was used in immunohistochemistry to investigate the mutations of ABCB4 in progressive familial intrahepatic cholestasis type 3 patients
|Giovannoni I,Santorelli FM,Candusso M,Di Rocco M,Bellacchio E,Callea F,Francalanci P||Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (43:567)||2011|
Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.
MA5-11986 was used in immunohistochemistry to investigate deciduoid features of malignant mesothelioma by histomorphological and immunohistochemical evaluation
|Ustun H,Astarci HM,Sungu N,Ozdemir A,Ekinci C||Diagnostic cytopathology (39:402)||2011|
Mucinous tubular and spindle cell carcinoma of kidney and problems in diagnosis.
MA5-11986 was used in immunohistochemistry to study problems in diagnosing mucinous tubular and spindle cell carcinoma of kidney
|Sarsik B,S¿m¿¿r A,Karaarslan S,Sen S||Turk patoloji dergisi (27:116)||2011|
A new method for morphometric analysis of tissue distribution of mobile cells in relation to immobile tissue structures.
MA5-11986 was used in immunohistochemistry to evaluate a new morphometric analysis method for cell mobility
|Nikitina L,Ahammer H,Blaschitz A,Gismondi A,Glasner A,Schimek MG,Dohr G,Sedlmayr P||PloS one (6:null)||2011|
The art of identification of extravillous trophoblast.
MA5-11986 was used in immunohistochemistry to study the difficulties in identification of extravillous trophoblast
|Moser G,Orendi K,Gauster M,Siwetz M,Helige C,Huppertz B||Placenta (32:197)||2011|
Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1, napsin A, p63, and CK5/6.
MA5-11986 was used in immunohistochemistry to study the utility of an immunohistochemical panel in subtyping non-small cell lung carcinomas lacking morphologic differentiation
|Mukhopadhyay S,Katzenstein AL||The American journal of surgical pathology (35:15)||2011|
In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands.
MA5-11986 was used in immunohistochemistry to investigate the integration of sweat glands into engineered skin constructs in vitro and in vivo in order to improve the quality of tissue regeneration
|Huang S,Xu Y,Wu C,Sha D,Fu X||Biomaterials (31:5520)||2010|
Lung surfactant proteins in the early human placenta.
MA5-11986 was used in immunohistochemistry to investigate the distribution of lung surfactant proteins in early human placenta
|Sati L,Seval-Celik Y,Demir R||Histochemistry and cell biology (133:85)||2010|
Giant cell tumor-like lesion of the urinary bladder: a report of two cases and literature review; giant cell tumor or undifferentiated carcinoma?
MA5-11986 was used in immunohistochemistry to report on two cases of giant cell tumor-like lesion of the urinary bladder
|Behzato¿lu K,Durak H,Canberk S,Aydin O,Huq GE,Oznur M,Ozyalvaçli G,Yildiz P||Diagnostic pathology (4:null)||2009|
Tumourigenic effect of Schistosoma haematobium total antigen in mammalian cells.
MA5-11986 was used in immunohistochemistry to investigate the influence of S. haematobium total antigen on tumor development
|Botelho M,Oliveira P,Gomes J,Gartner F,Lopes C,da Costa JM,Machado JC||International journal of experimental pathology (90:448)||2009|
Antigen expression of human eccrine sweat glands.
MA5-11986 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands
|Li HH,Zhou G,Fu XB,Zhang L||Journal of cutaneous pathology (36:318)||2009|
Chondromatous differentiation in clear cell carcinoma of the ovary: a rare finding challenging the differential diagnosis.
MA5-11986 was used in immunohistochemistry to investigate a rare case of chondromatous differentiation in an ovarian clear cell carcinoma
|Akbulut M,Zekioglu O,Terek MC,Ozdemir N||Archives of gynecology and obstetrics (278:569)||2008|
Arrangement of collagen fibers in human placental stem villi.
MA5-11986 was used in immunohistochemistry to investigate the organization of collagen fiber in human placental stem villi
|Sati L,Demir AY,Sarikcioglu L,Demir R||Acta histochemica (110:371)||2008|
Mucoepidermoid carcinoma of the parotid gland with ovarian and peritoneal metastasis.
MA5-11986 was used in immunohistochemistry to investigate the metastasis of mucoepidermoid carcinoma of the parotid gland
|Buyukkurt S,Altintas A,Gumurdulu D,Zeren H,Guzel B||The journal of obstetrics and gynaecology research (34:271)||2008|
Combination chemotherapy with gemcitabine and cisplatin as first-line treatment for immunohistochemically proven cholangiocarcinoma.
MA5-11986 was used in immunohistochemistry to investigate the combination chemotherapy of gemcitabine and cisplatin against cholangiocarcinoma
|Lee GW,Kang JH,Kim HG,Lee JS,Lee JS,Jang JS||American journal of clinical oncology (29:127)||2006|
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
MA5-11986 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver
|Yesim G,Gupse T,Zafer U,Ahmet A||Journal of hepato-biliary-pancreatic surgery (12:502)||2005|
Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays.
MA5-11986 was used in immunohistochemistry to establish the protein expression profiles for lung neoplasms
|Ullmann R,Morbini P,Halbwedl I,Bongiovanni M,Gogg-Kammerer M,Papotti M,Gabor S,Renner H,Popper HH||The Journal of pathology (203:798)||2004|
Isolated idiopathic bile ductular hyperplasia in patients with persistently abnormal liver function tests.
MA5-11986 was used in immunohistochemistry to characterize idiopathic isolated ductular hyperplasia in patients undergoing liver biopsy
|Sonzogni A,Colloredo G,Fabris L,Cadamuro M,Paris B,Roffi L,Pozzi M,Bovo G,Del Poggio P,Portmann BC,Strazzabosco M||Journal of hepatology (40:592)||2004|
CDX-2 homeobox gene expression is a reliable marker of colorectal adenocarcinoma metastases to the lungs.
MA5-11986 was used in immunohistochemistry to investigate the diagnostic value of CDX-2 in lung metastases from colorectal carcinomas
|Barbareschi M,Murer B,Colby TV,Chilosi M,Macri E,Loda M,Doglioni C||The American journal of surgical pathology (27:141)||2003|
Novel high-grade serous epithelial ovarian cancer cell lines that reflect the molecular diversity of both the sporadic and hereditary disease.
MA5-11986 was used in immunohistochemistry - paraffin section and western blot to analyze high-grade serous epithelial ovarian cancer cell lines that show sporadic and hereditary disease and the molecular diversity
|Fleury H,Communal L,Carmona E,Portelance L,Arcand SL,Rahimi K,Tonin PN,Provencher D,Mes-Masson AM||Genes and cancer (6:378)||2015|
Signet-ring cell carcinoma of the breast: a case report.
MA5-11986 was used in immunohistochemistry - paraffin section to present the case of a 46-year-old woman with primary signet-ring cell carcinoma of the breast
|Li X,Feng YF,Wei WD,Liu P,Xie ZM,Wang J,Xie XM||World journal of surgical oncology (11:null)||2013|
Stromal-epithelial crosstalk provides a suitable microenvironment for the progression of ovarian cancer cells in vitro.
MA5-11986 was used in immunocytochemistry to study the in vivo progression of ovarian cancer cells and the role of stromal-epithelial interactions in creating an appropriate microenvironment
|Fu S,Dong L,Sun W,Xu Y,Gao L,Miao Y||Cancer investigation (31:616)||2013|
Fusion of villous trophoblast can be visualized by localizing active caspase 8.
MA5-11986 was used in immunocytochemistry to examine the change of caspase 8 during the fusion of villous trophoblast
|Gauster M,Siwetz M,Huppertz B||Placenta (30:547)||2009|
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
MA5-11986 was used in immunocytochemistry to characterize nonparenchymal epithelial cells from adult human liver
|Duret C,Gerbal-Chaloin S,Ramos J,Fabre JM,Jacquet E,Navarro F,Blanc P,Sa-Cunha A,Maurel P,Daujat-Chavanieu M||Stem cells (Dayton, Ohio) (25:1779)||2007|
Proteomic analysis of zebrafish caudal fin regeneration.
MA5-11986 was used in western blot to study the time-course of zebrafish caudal fin regeneration at the proteomic level
|Saxena S,Singh SK,Lakshmi MG,Meghah V,Bhatti B,Swamy CV,Sundaram CS,Idris MM||Molecular and cellular proteomics : MCP (11:null)||2012|
Expression and distribution of nucleoside transporter proteins in the human syncytiotrophoblast.
MA5-11986 was used in immunohistochemistry to determine the localization and function of nucleoside transporter proteins in human syncytiotrophoblast from term placenta.
|Errasti-Murugarren E,Díaz P,Godoy V,Riquelme G,Pastor-Anglada M||Molecular pharmacology (80:809)||2011|