|Tested species reactivity||Human|
|Published species reactivity||Fish, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06316 detects Cytokeratin 7 in human samples.
MA1-06316 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry, and immunohistochemistry. Pretreatment is required for paraffin sections; 0.1% pronase/0.1% trypsine in PBS or 0.5% pepsine in HCL or microwave pretreatment suggested.
The MA1-06316 immunogen is a cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.
Store at 4°C, or in small aliquots at -20°C.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Serous carcinoma arising from uterine adenomyosis/adenomyotic cyst of the cervical stump: a report of 3 cases.
MA1-06316 was used in immunohistochemistry to study 3 cases of uterine adenomyosis/adenomyotic cysts of the cervical stump leading to serous carcinoma
|Lu B,Chen Q,Zhang X,Cheng L||Diagnostic pathology (11:null)||2016|
|Not Applicable||Not Cited||
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
MA1-06316 was used in immunohistochemistry to study a northern New England hospital and increased utilization, verification, and clinical implications of immunocytochemistry
|Sauter JL,Ambaye AB,Mount SL||Diagnostic cytopathology (43:688)||2015|
New diagnostic markers in salivary gland tumors.
MA1-06316 was used in immunohistochemistry to study the expresion of tumor antigens in order to identify novel diagnostic markers of parotid gland tumors
|Schneider S,Kloimstein P,Pammer J,Brannath W,Grasl MCh,Erovic BM||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (271:1999)||2014|
Amnion-derived mesenchymal stromal cells show a mesenchymal-epithelial phenotype in culture.
MA1-06316 was used in immunohistochemistry to study the mesenchymal-epithelial phenotype displayed by cultured mesenchymal stromal cells derived from the amniotic membrane
|König J,Lang I,Siwetz M,Fröhlich J,Huppertz B||Cell and tissue banking (15:193)||2014|
Tumor-induced STAT3 activation in monocytic myeloid-derived suppressor cells enhances stemness and mesenchymal properties in human pancreatic cancer.
MA1-06316 was used in immunohistochemistry to study the mechanism by which STAT3 activation promotes stemness in pancreatic cancer cancer stem cells
|Panni RZ,Sanford DE,Belt BA,Mitchem JB,Worley LA,Goetz BD,Mukherjee P,Wang-Gillam A,Link DC,Denardo DG,Goedegebuure SP,Linehan DC||Cancer immunology, immunotherapy : CII (63:513)||2014|
Three-dimensional culture and identification of human eccrine sweat glands in matrigel basement membrane matrix.
MA1-06316 was used in immunohistochemistry to develop a matrigel-based system for the 3-dimensional culture of human eccrine sweat glands
|Li H,Chen L,Zhang M,Tang S,Fu X||Cell and tissue research (354:897)||2013|
Identification of novel target proteins in sebaceous gland carcinoma.
MA1-06316 was used in immunohistochemistry to identify novel therapeutic target proteins in sebaceous gland carcinoma
|Erovic BM,Al Habeeb A,Harris L,Goldstein DP,Kim D,Ghazarian D,Irish JC||Head and neck (35:642)||2013|
Specimen quality evaluation in Canadian biobanks participating in the COEUR repository.
MA1-06316 was used in immunohistochemistry to study the quality of biological samples held in Canadian biobanks forming part of the COEUR repository
|Le Page C,Köbel M,de Ladurantaye M,Rahimi K,Madore J,Babinszky S,Bachvarov DR,Bachvarova M,Beauchamp MC,Cass CE,Chadwick D,Colleen C,Damaraju S,Dufour J,Gotlieb WH,Kalloger SE,Portelance L,McAlpine JN,Matte I,Piché A,Shaw P,Roehrl MH,Vanderhyden BC,Watson PH,Huntsman DG,Provencher DM,Mes-Masson AM||Biopreservation and biobanking (11:83)||2013|
Serrated pathway adenocarcinomas: molecular and immunohistochemical insights into their recognition.
MA1-06316 was used in immunohistochemistry to establish criteria for the recognition of serrated pathway adenocarcinomas
|Gurzu S,Szentirmay Z,Toth E,Bara T,Bara T,Jung I||PloS one (8:null)||2013|
Adenocarcinoma with sarcomatous dedifferentiation arising from mature cystic teratoma of the anterior mediastinum.
MA1-06316 was used in immunohistochemistry to report on a case of adenocarcinoma with sarcomatous dedifferentiation arising from mature cystic teratoma of the anterior mediastinum
|Kim JY,Lee CH,Park WY,Kim JY,Kim AR,Shin N,Park DY,Huh GY||Pathology, research and practice (208:741)||2012|
Biomarkers of parathyroid carcinoma.
MA1-06316 was used in immunohistochemistry to establish a biomarker panel suitable for the identification of parathyroid carcinomas
|Erovic BM,Harris L,Jamali M,Goldstein DP,Irish JC,Asa SL,Mete O||Endocrine pathology (23:221)||2012|
Miliary brain metastases from occult lung adenocarcinoma: Radiologic and histopathologic confirmation.
MA1-06316 was used in immunohistochemistry to report on a case of miliary brain metastases from occult lung adenocarcinoma
|Kahveci R,Gürer B,Kaygusuz G,Sekerci Z||Journal of neurosciences in rural practice (3:386)||2012|
Significance of TRIM29 and ß-catenin expression in non-small-cell lung cancer.
MA1-06316 was used in immunohistochemistry to study non-small cell lung cancer and the potential clinicopathological significance of TRIM29 and beta-catenin expression
|Zhou ZY,Yang GY,Zhou J,Yu MH||Journal of the Chinese Medical Association : JCMA (75:269)||2012|
A new case of primary signet-ring cell carcinoma of the cervix with prominent endometrial and myometrial involvement: Immunohistochemical and molecular studies and review of the literature.
MA1-06316 was used in immunohistochemistry to report on a case of primary signet-ring cell carcinoma of the cervix with endometrial and myometrial involvement
|Giordano G,Pizzi S,Berretta R,D'Adda T||World journal of surgical oncology (10:null)||2012|
Aberrant pattern of the cytokeratin 7/cytokeratin 20 immunophenotype in colorectal adenocarcinomas with BRAF mutations.
MA1-06316 was used in immunohistochemistry to study the aberrant cytokeratin 7/cytokeratin 20 immunophenotype in colorectal adenocarcinomas with BRAF mutations
|Gurzu S,Jung I||Pathology, research and practice (208:163)||2012|
The value of CDX2 and cytokeratins 7 and 20 expression in differentiating colorectal adenocarcinomas from extraintestinal gastrointestinal adenocarcinomas: cytokeratin 7-/20+ phenotype is more specific than CDX2 antibody.
MA1-06316 was used in immunohistochemistry to study the value of CDX2 and cytokeratin 7 and 20 expression in differentiating colorectal adenocarcinomas from extraintestinal gastrointestinal adenocarcinomas
|Bayrak R,Haltas H,Yenidunya S||Diagnostic pathology (7:null)||2012|
Two novel mutations in African and Asian children with progressive familial intrahepatic cholestasis type 3.
MA1-06316 was used in immunohistochemistry to investigate the mutations of ABCB4 in progressive familial intrahepatic cholestasis type 3 patients
|Giovannoni I,Santorelli FM,Candusso M,Di Rocco M,Bellacchio E,Callea F,Francalanci P||Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver (43:567)||2011|
Mucinous tubular and spindle cell carcinoma of kidney and problems in diagnosis.
MA1-06316 was used in immunohistochemistry to study problems in diagnosing mucinous tubular and spindle cell carcinoma of kidney
|Sarsik B,S¿m¿¿r A,Karaarslan S,Sen S||Turk patoloji dergisi (27:116)||2011|
The art of identification of extravillous trophoblast.
MA1-06316 was used in immunohistochemistry to study the difficulties in identification of extravillous trophoblast
|Moser G,Orendi K,Gauster M,Siwetz M,Helige C,Huppertz B||Placenta (32:197)||2011|
Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1, napsin A, p63, and CK5/6.
MA1-06316 was used in immunohistochemistry to study the utility of an immunohistochemical panel in subtyping non-small cell lung carcinomas lacking morphologic differentiation
|Mukhopadhyay S,Katzenstein AL||The American journal of surgical pathology (35:15)||2011|
In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands.
MA1-06316 was used in immunohistochemistry to investigate the integration of sweat glands into engineered skin constructs in vitro and in vivo in order to improve the quality of tissue regeneration
|Huang S,Xu Y,Wu C,Sha D,Fu X||Biomaterials (31:5520)||2010|
Immunohistochemical comparative study of fibrosis and biliary ductular reaction in alcoholic and viral chronic hepatitis.
MA1-06316 was used in immunohistochemistry to investigate the characteristics of fibrosis in patients with alcoholic and viral chronic hepatitis
|Egyed-Zsigmond I,Jung I,Egyed-Zsigmond I,Marton G,Gurzu S,Mezei T||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (51:265)||2010|
Lung surfactant proteins in the early human placenta.
MA1-06316 was used in immunohistochemistry to investigate the distribution of lung surfactant proteins in early human placenta
|Sati L,Seval-Celik Y,Demir R||Histochemistry and cell biology (133:85)||2010|
Giant cell tumor-like lesion of the urinary bladder: a report of two cases and literature review; giant cell tumor or undifferentiated carcinoma?
MA1-06316 was used in immunohistochemistry to report on two cases of giant cell tumor-like lesion of the urinary bladder
|Behzato¿lu K,Durak H,Canberk S,Aydin O,Huq GE,Oznur M,Ozyalvaçli G,Yildiz P||Diagnostic pathology (4:null)||2009|
Antigen expression of human eccrine sweat glands.
MA1-06316 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands
|Li HH,Zhou G,Fu XB,Zhang L||Journal of cutaneous pathology (36:318)||2009|
Chondromatous differentiation in clear cell carcinoma of the ovary: a rare finding challenging the differential diagnosis.
MA1-06316 was used in immunohistochemistry to investigate a rare case of chondromatous differentiation in an ovarian clear cell carcinoma
|Akbulut M,Zekioglu O,Terek MC,Ozdemir N||Archives of gynecology and obstetrics (278:569)||2008|
Arrangement of collagen fibers in human placental stem villi.
MA1-06316 was used in immunohistochemistry to investigate the organization of collagen fiber in human placental stem villi
|Sati L,Demir AY,Sarikcioglu L,Demir R||Acta histochemica (110:371)||2008|
Mucoepidermoid carcinoma of the parotid gland with ovarian and peritoneal metastasis.
MA1-06316 was used in immunohistochemistry to investigate the metastasis of mucoepidermoid carcinoma of the parotid gland
|Buyukkurt S,Altintas A,Gumurdulu D,Zeren H,Guzel B||The journal of obstetrics and gynaecology research (34:271)||2008|
Combination chemotherapy with gemcitabine and cisplatin as first-line treatment for immunohistochemically proven cholangiocarcinoma.
MA1-06316 was used in immunohistochemistry to investigate the combination chemotherapy of gemcitabine and cisplatin against cholangiocarcinoma
|Lee GW,Kang JH,Kim HG,Lee JS,Lee JS,Jang JS||American journal of clinical oncology (29:127)||2006|
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
MA1-06316 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver
|Yesim G,Gupse T,Zafer U,Ahmet A||Journal of hepato-biliary-pancreatic surgery (12:502)||2005|
Protein expression profiles in adenocarcinomas and squamous cell carcinomas of the lung generated using tissue microarrays.
MA1-06316 was used in immunohistochemistry to establish the protein expression profiles for lung neoplasms
|Ullmann R,Morbini P,Halbwedl I,Bongiovanni M,Gogg-Kammerer M,Papotti M,Gabor S,Renner H,Popper HH||The Journal of pathology (203:798)||2004|
Isolated idiopathic bile ductular hyperplasia in patients with persistently abnormal liver function tests.
MA1-06316 was used in immunohistochemistry to characterize idiopathic isolated ductular hyperplasia in patients undergoing liver biopsy
|Sonzogni A,Colloredo G,Fabris L,Cadamuro M,Paris B,Roffi L,Pozzi M,Bovo G,Del Poggio P,Portmann BC,Strazzabosco M||Journal of hepatology (40:592)||2004|
CDX-2 homeobox gene expression is a reliable marker of colorectal adenocarcinoma metastases to the lungs.
MA1-06316 was used in immunohistochemistry to investigate the diagnostic value of CDX-2 in lung metastases from colorectal carcinomas
|Barbareschi M,Murer B,Colby TV,Chilosi M,Macri E,Loda M,Doglioni C||The American journal of surgical pathology (27:141)||2003|
Stromal-epithelial crosstalk provides a suitable microenvironment for the progression of ovarian cancer cells in vitro.
MA1-06316 was used in immunocytochemistry to study the in vivo progression of ovarian cancer cells and the role of stromal-epithelial interactions in creating an appropriate microenvironment
|Fu S,Dong L,Sun W,Xu Y,Gao L,Miao Y||Cancer investigation (31:616)||2013|
Fusion of villous trophoblast can be visualized by localizing active caspase 8.
MA1-06316 was used in immunocytochemistry to examine the change of caspase 8 during the fusion of villous trophoblast
|Gauster M,Siwetz M,Huppertz B||Placenta (30:547)||2009|
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
MA1-06316 was used in immunocytochemistry to characterize nonparenchymal epithelial cells from adult human liver
|Duret C,Gerbal-Chaloin S,Ramos J,Fabre JM,Jacquet E,Navarro F,Blanc P,Sa-Cunha A,Maurel P,Daujat-Chavanieu M||Stem cells (Dayton, Ohio) (25:1779)||2007|
Signet-ring cell carcinoma of the breast: a case report.
MA1-06316 was used in immunohistochemistry - paraffin section to present the case of a 46-year-old woman with primary signet-ring cell carcinoma of the breast
|Li X,Feng YF,Wei WD,Liu P,Xie ZM,Wang J,Xie XM||World journal of surgical oncology (11:null)||2013|
Proteomic analysis of zebrafish caudal fin regeneration.
MA1-06316 was used in western blot to study the time-course of zebrafish caudal fin regeneration at the proteomic level
|Saxena S,Singh SK,Lakshmi MG,Meghah V,Bhatti B,Swamy CV,Sundaram CS,Idris MM||Molecular and cellular proteomics : MCP (11:null)||2012|
Expression and distribution of nucleoside transporter proteins in the human syncytiotrophoblast.
MA1-06316 was used in immunohistochemistry to determine the localization and function of nucleoside transporter proteins in human syncytiotrophoblast from term placenta.
|Errasti-Murugarren E,Díaz P,Godoy V,Riquelme G,Pastor-Anglada M||Molecular pharmacology (80:809)||2011|