|Tested species reactivity||Dog, Cat, Goat, Hamster, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratins from the human bladder carcinoma cell line T24.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100 - 1:200|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06315 detects cytokeratin 7 in human, mouse, rat, hamster, canine, feline, goat and zebrafish samples.
MA1-06315 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry and immunohistochemistry.
The MA1-06315 immunogens are cytokeratins from the human bladder carcinoma cell line T24.
Store at 4°C or in small aliquots at -20°C.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Microanatomy of the cervical and anorectal squamocolumnar junctions: a proposed model for anatomical differences in HPV-related cancer risk.
MA1-06315 was used in immunohistochemistry - paraffin section to discuss the different sites of human papilloma virus-induced cancer.
|Yang EJ,Quick MC,Hanamornroongruang S,Lai K,Doyle LA,McKeon FD,Xian W,Crum CP,Herfs M||Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (28:994)||2015|
A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.
MA1-06315 was used in immunohistochemistry to study the role of the cervical squamocolumnar junction in cervical remodeling and neoplasia
|Herfs M,Vargas SO,Yamamoto Y,Howitt BE,Nucci MR,Hornick JL,McKeon FD,Xian W,Crum CP||The Journal of pathology (229:460)||2013|
A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer.
MA1-06315 was used in immunohistochemistry to study the role of a discrete population of squamocolumnar junction cells in the pathogenesis of cervical cancer
|Herfs M,Yamamoto Y,Laury A,Wang X,Nucci MR,McLaughlin-Drubin ME,Münger K,Feldman S,McKeon FD,Xian W,Crum CP||Proceedings of the National Academy of Sciences of the United States of America (109:10516)||2012|
Understanding the role of keratins 8 and 18 in neoplastic potential of breast cancer derived cell lines.
MA1-06315 was used in western blot to assess the use of K8/18 in breast cancer prognosis
|Iyer SV,Dange PP,Alam H,Sawant SS,Ingle AD,Borges AM,Shirsat NV,Dalal SN,Vaidya MM||PloS one (8:null)||2013|