Immunofluorescent analysis of Cytokeratin 8, 18 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8, 18 (5D3) Mouse Monoclonal Antibody (MA5-14088) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratins purified from breast cancer MCF-7 cells|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14088 targets Cytokeratin 8/18 in immunohistochemistry (paraffin) applications and shows reactivity with Human samples.
The MA5-14088 immunogen is cytokeratins purified from breast cancer MCF-7 cells.
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis.
MA5-14088 was used in immunocytochemistry to research chlamydia trachomatis and infection of human retinal pigment epithelium
|Boiko E,Maltsev D,Savicheva A,Shalepo K,Khusnutdinova T,Pozniak A,Kvetnoi I,Polyakova V,Suetov A||PloS one (10:null)||2015|
Parietal epithelia cells in the urine as a marker of disease activity in glomerular diseases.
MA5-14088 was used in immunocytochemistry to evaluate the presence of PDX-positive cells in urine as a marker for glomerular diseases
|Achenbach J,Mengel M,Tossidou I,Peters I,Park JK,Haubitz M,Ehrich JH,Haller H,Schiffer M||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (23:3138)||2008|
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
MA5-14088 was used in immunocytochemistry to characterize nonparenchymal epithelial cells from adult human liver
|Duret C,Gerbal-Chaloin S,Ramos J,Fabre JM,Jacquet E,Navarro F,Blanc P,Sa-Cunha A,Maurel P,Daujat-Chavanieu M||Stem cells (Dayton, Ohio) (25:1779)||2007|
A reference image-based method for optimization of clinical immunohistochemistry.
MA5-14088 was used in immunohistochemistry to describe a new method for immunohistochemistry optimization
|Kap M,Lam KH,Ewing-Graham P,Riegman P||Histopathology (67:193)||2015|
Pancreatic mucinous cystic tumor in Turner syndrome: How a tumor bends to a genetic disease.
MA5-14088 was used in immunohistochemistry to report on a case of pancreatic mucinous cystic tumor in a patient with Turner syndrome
|Pizzi M,Pennelli G,Merante-Boschin I,Fassan M,Pelizzo MR,Rugge M||International journal of surgery case reports (4:1028)||2013|
Primary intracranial Ewing sarcoma with an unusually aggressive course: a case report and review of the literature.
MA5-14088 was used in immunohistochemistry to report on a case of primary intracranial Ewing sarcoma with an unusually aggressive course
|Tanboon J,Sitthinamsuwan B,Paruang T,Marrano P,Thorner PS||Neuropathology : official journal of the Japanese Society of Neuropathology (32:293)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA5-14088 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Primary sellar neuroblastoma presenting with syndrome of inappropriate secretion of anti-diuretic hormone.
MA5-14088 was used in immunohistochemistry to investigate the secretion of anti-diuretic hormone in neuroblastoma
|Radotra B,Apostolopoulos V,Sandison A,Hatfield EC,Mendoza N,Moss J,Mehta A,Glaser M,Meeran K,Roncaroli F||Endocrine pathology (21:266)||2010|
Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-like cells.
MA5-14088 was used in immunohistochemistry to investigate the culture and differentiation of human liver progenitor cells
|Gerbal-Chaloin S,Duret C,Raulet E,Navarro F,Blanc P,Ramos J,Maurel P,Daujat-Chavanieu M||Methods in molecular biology (Clifton, N.J.) (640:247)||2010|
An immunohistochemical profile of giant cell carcinoma of the larynx.
MA5-14088 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx
|Gurbuz Y,Kose N,Aydin O,Ozturk M||Auris, nasus, larynx (34:413)||2007|
Breast cancer in an MSH2 gene mutation carrier.
MA5-14088 was used in immunohistochemistry to report on a case of breast cancer in a MSH2 gene mutation carrier
|Westenend PJ,Schütte R,Hoogmans MM,Wagner A,Dinjens WN||Human pathology (36:1322)||2005|
An unusual presentation of Helicobacter pylori infection: so-called "Russell body gastritis".
MA5-14088 was used in immunohistochemistry to report on a case of Helicobacter pylori infection with Russell body involvement
|Ensari A,Savas B,Okcu Heper A,Kuzu I,Idilman R||Virchows Archiv : an international journal of pathology (446:463)||2005|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA5-14088 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Histological assessment of PAXgene tissue fixation and stabilization reagents.
MA5-14088 was used in immunohistochemistry - paraffin section to examine the suitability of PAXgene tissue fixation for (immuno)histological methods
|Kap M,Smedts F,Oosterhuis W,Winther R,Christensen N,Reischauer B,Viertler C,Groelz D,Becker KF,Zatloukal K,Langer R,Slotta-Huspenina J,Bodo K,de Jong B,Oelmuller U,Riegman P||PloS one (6:null)||2011|