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Immunofluorescent analysis of Cytokeratin 8, 18 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8, 18 (5D3) Mouse Monoclonal Antibody (MA5-14088) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratins purified from breast cancer MCF-7 cells|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14088 targets Cytokeratin 8/18 in immunohistochemistry (paraffin) applications and shows reactivity with Human samples.
The MA5-14088 immunogen is cytokeratins purified from breast cancer MCF-7 cells.
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A reference image-based method for optimization of clinical immunohistochemistry.
MA5-14088 was used in immunohistochemistry to describe a new method for immunohistochemistry optimization
|Kap M,Lam KH,Ewing-Graham P,Riegman P||Histopathology (67:193)||2015|
Pancreatic mucinous cystic tumor in Turner syndrome: How a tumor bends to a genetic disease.
MA5-14088 was used in immunohistochemistry to report on a case of pancreatic mucinous cystic tumor in a patient with Turner syndrome
|Pizzi M,Pennelli G,Merante-Boschin I,Fassan M,Pelizzo MR,Rugge M||International journal of surgery case reports (4:1028)||2013|
Primary intracranial Ewing sarcoma with an unusually aggressive course: a case report and review of the literature.
MA5-14088 was used in immunohistochemistry to report on a case of primary intracranial Ewing sarcoma with an unusually aggressive course
|Tanboon J,Sitthinamsuwan B,Paruang T,Marrano P,Thorner PS||Neuropathology : official journal of the Japanese Society of Neuropathology (32:293)||2012|
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
MA5-14088 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression
|Yin WH,Li J,Chan JK||Diagnostic pathology (7:null)||2012|
Primary sellar neuroblastoma presenting with syndrome of inappropriate secretion of anti-diuretic hormone.
MA5-14088 was used in immunohistochemistry to investigate the secretion of anti-diuretic hormone in neuroblastoma
|Radotra B,Apostolopoulos V,Sandison A,Hatfield EC,Mendoza N,Moss J,Mehta A,Glaser M,Meeran K,Roncaroli F||Endocrine pathology (21:266)||2010|
Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-like cells.
MA5-14088 was used in immunohistochemistry to investigate the culture and differentiation of human liver progenitor cells
|Gerbal-Chaloin S,Duret C,Raulet E,Navarro F,Blanc P,Ramos J,Maurel P,Daujat-Chavanieu M||Methods in molecular biology (Clifton, N.J.) (640:247)||2010|
An immunohistochemical profile of giant cell carcinoma of the larynx.
MA5-14088 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx
|Gurbuz Y,Kose N,Aydin O,Ozturk M||Auris, nasus, larynx (34:413)||2007|
Breast cancer in an MSH2 gene mutation carrier.
MA5-14088 was used in immunohistochemistry to report on a case of breast cancer in a MSH2 gene mutation carrier
|Westenend PJ,Schütte R,Hoogmans MM,Wagner A,Dinjens WN||Human pathology (36:1322)||2005|
An unusual presentation of Helicobacter pylori infection: so-called "Russell body gastritis".
MA5-14088 was used in immunohistochemistry to report on a case of Helicobacter pylori infection with Russell body involvement
|Ensari A,Savas B,Okcu Heper A,Kuzu I,Idilman R||Virchows Archiv : an international journal of pathology (446:463)||2005|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA5-14088 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Parietal epithelia cells in the urine as a marker of disease activity in glomerular diseases.
MA5-14088 was used in immunocytochemistry to evaluate the presence of PDX-positive cells in urine as a marker for glomerular diseases
|Achenbach J,Mengel M,Tossidou I,Peters I,Park JK,Haubitz M,Ehrich JH,Haller H,Schiffer M||Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (23:3138)||2008|
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
MA5-14088 was used in immunocytochemistry to characterize nonparenchymal epithelial cells from adult human liver
|Duret C,Gerbal-Chaloin S,Ramos J,Fabre JM,Jacquet E,Navarro F,Blanc P,Sa-Cunha A,Maurel P,Daujat-Chavanieu M||Stem cells (Dayton, Ohio) (25:1779)||2007|
cell proliferation-inducing gene 46 protein; CK-8; cytokeratin 18; cytokeratin 8; cytokeratin-8; keratin; keratin 18, type I; keratin 8, type II; keratin-8; type II cytoskeletal 8; type-II keratin Kb8
CARD2; CK-18; CK-8; CK8; CYK18; CYK8; K18; K2C8; K8; KO; KRT18; KRT8; PIG46