|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA5-15460 targets Cytokeratin 8 in IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15460 immunogen is purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.
MA5-15460 detects Cytokeratin 8 which has a predicted molecular weight of approximately 54kDa.
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The cultural divide: exponential growth in classical 2D and metabolic equilibrium in 3D environments.
MA5-15460 was used in immunocytochemistry to analyze the proteomes of 2D and 3D cell cultures
|Wrzesinski K,Rogowska-Wrzesinska A,Kanlaya R,Borkowski K,Schwämmle V,Dai J,Joensen KE,Wojdyla K,Carvalho VB,Fey SJ||PloS one (9:null)||2014|