Immunofluorescence analysis of Cytokeratin 8 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8 Monoclonal Antibody (Product # MA5-14476) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to residues of the human CK18|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14476 targets Cytokeratin 8 in IHC (P) applications and shows reactivity with Human samples.
The MA5-14476 immunogen is a synthetic peptide corresponding to residues of the human CK18.
Cytokeratin 8 belongs to the type B (basic) subfamily of low molecular weight keratins and usually exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous simple epithelia. It is expressed in mesothelioma and most adenocarcinomas of simple epithelium including breast ducts, gall bladder, intestine, liver, pancreas and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool.
MA5-14476 was used in immunohistochemistry - paraffin section to describe an automated image method capable of discriminating epithelial and stromal areas of colorectal cancer samples
|Rogojanu R,Thalhammer T,Thiem U,Heindl A,Mesteri I,Seewald A,Jäger W,Smochina C,Ellinger I,Bises G||BioMed research international (2015:null)||2015|
Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.
MA5-14476 was used in immunocytochemistry and western blot to study the opposing effects of hyperglycemia and SIRT2 on keratin-8 lysine acetylation
|Snider NT,Leonard JM,Kwan R,Griggs NW,Rui L,Omary MB||The Journal of cell biology (200:241)||2013|
Characterization of the expression of cytokeratins 5, 8, and 14 in mouse thymic epithelial cells during thymus regeneration following acute thymic involution.
MA5-14476 was used in immunohistochemistry to profile cytokeratin expression in murine thymic epithelial cells during thymus regeneration
|Lee EN,Park JK,Lee JR,Oh SO,Baek SY,Kim BS,Yoon S||Anatomy and cell biology (44:14)||2011|