|Tested species reactivity||Human, Rabbit, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Keratin isolated from the human breast carcinoma cell line MCF-7.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-06318 detects cytokeratin 8 in rat, human, and rabbit samples.
MA1-06318 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry and immunohistochemistry (frozen, paraffin).
The MA1-06318 immunogen is keratin isolated from the human breast carcinoma cell line MCF-7.
Store at 4°C or in small aliquots at -20°C.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Keratin 8 phosphorylation regulates its transamidation and hepatocyte Mallory-Denk body formation.
MA1-06318 was used in western blot to study the role of keratin 8 phosphorylation in regulating Mallory-Denk body formation
|Kwan R,Hanada S,Harada M,Strnad P,Li DH,Omary MB||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (26:2318)||2012|
Analysis of keratin polypeptides 8 and 19 variants in inflammatory bowel disease.
MA1-06318 was used in western blot to study whether variants of keratin 8 and keratin 19 are associated with inflammatory bowel disease
|Tao GZ,Strnad P,Zhou Q,Kamal A,Zhang L,Madani ND,Kugathasan S,Brant SR,Cho JH,Omary MB,Duerr RH||Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association (5:857)||2007|
Protein phosphatase-2A associates with and dephosphorylates keratin 8 after hyposmotic stress in a site- and cell-specific manner.
MA1-06318 was used in western blot to study the site-specific dephosphorylation of keratin 8 by protein phosphatase-2A and how this varies according to cell type
|Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB||Journal of cell science (119:1425)||2006|
Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies.
MA1-06318 was used in western blot to produce and characterize human disease-related anti-keratin rabbit monoclonal antibodies
|Tao GZ,Nakamichi I,Ku NO,Wang J,Frolkis M,Gong X,Zhu W,Pytela R,Omary MB||Experimental cell research (312:411)||2006|
Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease.
MA1-06318 was used in western blot to study keratin 8 and keratin 18 hyperphosphorylation as markers for progression of human liver disease
|Toivola DM,Ku NO,Resurreccion EZ,Nelson DR,Wright TL,Omary MB||Hepatology (Baltimore, Md.) (40:459)||2004|
Keratin 8 mutations in patients with cryptogenic liver disease.
MA1-06318 was used in western blot to study mutations of keratin 8 in cryptogenic liver disease patients
|Ku NO,Gish R,Wright TL,Omary MB||The New England journal of medicine (344:1580)||2001|
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
MA1-06318 was used in immunocytochemistry to investigate the transdifferentiation of human embryonic pancreatic cells to hepatocytes
|Sumitran-Holgersson S,Nowak G,Thowfeequ S,Begum S,Joshi M,Jaksch M,Kjaeldgaard A,Jorns C,Ericzon BG,Tosh D||Cell transplantation (18:183)||2009|
Antigen expression of human eccrine sweat glands.
MA1-06318 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands
|Li HH,Zhou G,Fu XB,Zhang L||Journal of cutaneous pathology (36:318)||2009|
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
MA1-06318 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver
|Yesim G,Gupse T,Zafer U,Ahmet A||Journal of hepato-biliary-pancreatic surgery (12:502)||2005|