|Flow Cytometry (Flow)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100-1:1000|
|Western Blot (WB)||See 6 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Tested Species reactivity||Human, Rabbit, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Keratin isolated from the human breast carcinoma cell line MCF-7.|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
MA1-06318 detects cytokeratin 8 in rat, human, and rabbit samples.
MA1-06318 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry and immunohistochemistry (frozen, paraffin).
The MA1-06318 immunogen is keratin isolated from the human breast carcinoma cell line MCF-7.
Store at 4°C or in small aliquots at -20°C.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CK-8; Cytokeratin endo A; Cytokeratin-8; K-8; K8; Keratin; keratin 8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; type II cytoskeletal 8; Type-II keratin Kb8
Gene Aliases: CARD2; CK-8; CK8; CYK8; CYKER; K2C8; K8; KO; Krt2-8; KRT8
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