|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 224 of Human Cytokeratin 8|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-28985 targets Cytokeratin 8 in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-28985 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 224 of Human Cytokeratin 8.
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Quantitative interactome analysis reveals a chemoresistant edgotype.
PA5-28985 was used in immunoprecipitation to use chemical crosslinking and mass spectrometry to quantify changes in protein structures and interactions in multidrug-resistant human carcinoma cells
|Chavez JD,Schweppe DK,Eng JK,Zheng C,Taipale A,Zhang Y,Takara K,Bruce JE||Nature communications (6:null)||2015|