Immunofluorescent analysis of Cytokeratin 8 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a Cytokeratin 8 polyclonal antibody (Product # PA5-34762) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 227 and 483 of Cytokeratin 8 (Uniprot ID#P05787)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34762 targets Cytokeratin 8 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and Mouse samples.
The PA5-34762 immunogen is recombinant fragment corresponding to a region within amino acids 227 and 483 of Cytokeratin 8 (Uniprot ID#P05787).
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.