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|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Keratin preparation from a human carcinoma|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14425 targets Cytokeratin 8 in IHC (P) and IP applications and shows reactivity with Human samples.
The MA5-14425 immunogen is keratin preparation from a human carcinoma.
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.
MA5-14425 was used in immunoprecipitation to study the opposing effects of hyperglycemia and SIRT2 on keratin-8 lysine acetylation
|Snider NT,Leonard JM,Kwan R,Griggs NW,Rui L,Omary MB||The Journal of cell biology (200:241)||2013|
Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.
MA5-14425 was used in western blot to study the effect of keratin 18 overexpression on the cell surface expression of the cystic fibrosis transmembrane conductance regulator
|Duan Y,Sun Y,Zhang F,Zhang WK,Wang D,Wang Y,Cao X,Hu W,Xie C,Cuppoletti J,Magin TM,Wang H,Wu Z,Li N,Huang P||The Journal of biological chemistry (287:40547)||2012|
Aging modulates susceptibility to mouse liver Mallory-Denk body formation.
MA5-14425 was used in western blot to study the effect of aging on Mallory-Denk body formation in mouse liver
|Hanada S,Harada M,Abe M,Akiba J,Sakata M,Kwan R,Taniguchi E,Kawaguchi T,Koga H,Nagata E,Ueno T,Sata M||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (60:475)||2012|
Identification of nm23-H1 as a metastatic suppressor and prognostic factor in nasopharyngeal carcinoma by proteomic analysis.
MA5-14425 was used in western blot to study the role of nm23-H1 as a suppressor of nasopharyngeal carcinoma metastasis and its value as a prognostic marker
|Li X,Hu R,Qu J,He Q,Chen Y,Li J,Ye X,Xiang Y,Yi H||Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (37:17)||2012|
Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells.
MA5-14425 was used in western blot to study the role of keratins in modulating cFlip and ERK1/2 apoptotic signaling in epithelial cells
|Gilbert S,Loranger A,Marceau N||Molecular and cellular biology (24:7072)||2004|
Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model.
MA5-14425 was used in immunohistochemistry and western blot to study the role of Hedgehog overexpression in the formation of prostate cancer stem cells with metastatic potential
|Chang HH,Chen BY,Wu CY,Tsao ZJ,Chen YY,Chang CP,Yang CR,Lin DP||Journal of biomedical science (18:null)||2011|
Molecular characterization of breast cancer in young Brazilian women.
MA5-14425 was used in immunohistochemistry to study the molecular features of breast cancer in young Brazilian women
|Carvalho LV,Pereira EM,Frappart L,Boniol M,Bernardo WM,Tarricone V,Tavtigian S,Southey MC||Revista da Associac¿a¿o Me¿dica Brasileira (1992) (56:278)||2010|
Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.
MA5-14425 was used in immunocytochemistry to study keratin hypersumoylation and its utility as a marker of human liver disease and mutation of keratin
|Snider NT,Weerasinghe SV,Iñiguez-Lluhí JA,Herrmann H,Omary MB||The Journal of biological chemistry (286:2273)||2011|
Functional testing of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex.
MA5-14425 was used in immunocytochemistry to study the function of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex
|Sørensen CB,Andresen BS,Jensen UB,Jensen TG,Jensen PK,Gregersen N,Bolund L||Experimental dermatology (12:472)||2003|
CK-8; cytokeratin 8; cytokeratin-8; keratin; keratin 8, type II; keratin-8; type II cytoskeletal 8; type-II keratin Kb8
CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; KRT8