Immunofluorescent analysis of Cytokeratin 8 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8 (TS1) Mouse Monoclonal Antibody (MA5-14428) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Keratin preparation from a human carcinoma|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14428 targets Cytokeratin 8 in IHC (P) and IP applications and shows reactivity with Human samples.
The MA5-14428 immunogen is keratin preparation from a human carcinoma.
Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report.
MA5-14428 was used in immunohistochemistry to describe a tumor from a patient with oncocytic cystadenoma
|Ba¿ak K,Kiro¿lu K||Medicine (93:null)||2014|
Molecular characterization of breast cancer in young Brazilian women.
MA5-14428 was used in immunohistochemistry to study the molecular features of breast cancer in young Brazilian women
|Carvalho LV,Pereira EM,Frappart L,Boniol M,Bernardo WM,Tarricone V,Tavtigian S,Southey MC||Revista da Associacao Medica Brasileira (1992) (56:278)||2010|
Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.
MA5-14428 was used in immunoprecipitation to study the opposing effects of hyperglycemia and SIRT2 on keratin-8 lysine acetylation
|Snider NT,Leonard JM,Kwan R,Griggs NW,Rui L,Omary MB||The Journal of cell biology (200:241)||2013|
Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.
MA5-14428 was used in western blot to study the effect of keratin 18 overexpression on the cell surface expression of the cystic fibrosis transmembrane conductance regulator
|Duan Y,Sun Y,Zhang F,Zhang WK,Wang D,Wang Y,Cao X,Hu W,Xie C,Cuppoletti J,Magin TM,Wang H,Wu Z,Li N,Huang P||The Journal of biological chemistry (287:40547)||2012|
Aging modulates susceptibility to mouse liver Mallory-Denk body formation.
MA5-14428 was used in western blot to study the effect of aging on Mallory-Denk body formation in mouse liver
|Hanada S,Harada M,Abe M,Akiba J,Sakata M,Kwan R,Taniguchi E,Kawaguchi T,Koga H,Nagata E,Ueno T,Sata M||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (60:475)||2012|
Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
MA5-14428 was used in western blot to examine the biological responses imparted by Matrigel overlays on primary human hepatocyte cultures
|Page JL,Johnson MC,Olsavsky KM,Strom SC,Zarbl H,Omiecinski CJ||Toxicological sciences : an official journal of the Society of Toxicology (97:384)||2007|
Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells.
MA5-14428 was used in western blot to study the role of keratins in modulating cFlip and ERK1/2 apoptotic signaling in epithelial cells
|Gilbert S,Loranger A,Marceau N||Molecular and cellular biology (24:7072)||2004|
Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.
MA5-14428 was used in immunocytochemistry to study keratin hypersumoylation and its utility as a marker of human liver disease and mutation of keratin
|Snider NT,Weerasinghe SV,Iñiguez-Lluhí JA,Herrmann H,Omary MB||The Journal of biological chemistry (286:2273)||2011|
Functional testing of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex.
MA5-14428 was used in immunocytochemistry to study the function of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex
|Sørensen CB,Andresen BS,Jensen UB,Jensen TG,Jensen PK,Gregersen N,Bolund L||Experimental dermatology (12:472)||2003|