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Product Details
MUB0318P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M20
Immunogen
Cytokeratin 8 / Keratin K8
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells and epithelia of the digestive, respiratory and urogenital tract. Also most adenocarcinomas derived from these cells are positive. The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of Cytokeratin 8 (see reference 10).
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CK-8; Cytokeratin endo A; Cytokeratin-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8
Gene Aliases: CARD2; CK-8; CK8; CYK8; CYKER; K2C8; K8; KO; Krt2-8; KRT8
UniProt ID: (Human) P05787, (Rat) Q10758
Entrez Gene ID: (Rabbit) 100348891, (Human) 3856, (Rat) 25626
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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