Immunofluorescence analysis of Cytokeratin HMW was done on 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with of Cytokeratin HMW (34BetaE12) Mouse Monoclonal Antibody (MA512135) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Horse, Human, Non-human primate, Rabbit|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Solubilized keratin extracted from human stratum corneum|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 12 publications below|
MA5-12135 targets Cytokeratin HMW in ICC/IF, WB and IHC (P) applications and shows reactivity with Equine, Human, Non-human primate, and Rabbit samples. This antibody does not react with rat tissue in Western blot applications.
The MA5-12135 immunogen is solubilized keratin extracted from human stratum corneum.
In normal cells, Ab-3 labels squamous, ductal and other complex epithelia. It reacts with benign small-acinir lesions of the prostate and does not react with hepatocytes, pancreatic acinar cells, proximal renal tubes or endometrial glands. In tumor cells, this antibody is reactive with both squamous and ductal neoplasms and variably with those derived from simple epithelium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Romanowsky-Giemsa as a counterstain for immunohistochemistry: optimizing a traditional reagent.
MA5-12135 was used in immunohistochemistry to develop a protocol for the counterstaining of immunohistochemical sections using Romanowsky-Giemsa
|Stefanovi¿ D,Stefanovi¿ M,Nikin Z||Biotechnic and histochemistry : official publication of the Biological Stain Commission (88:329)||2013|
Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.
MA5-12135 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population
|Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (121:272)||2013|
Sinonasal teratocarcinosarcoma: a clinical and pathological analysis.
MA5-12135 was used in immunohistochemistry to report on two cases of sinonasal teratocarcinosarcoma
|Yang S,Sun R,Liang J,Zhou Z,Zhou J,Rui J||International journal of surgical pathology (21:37)||2013|
Chemical castration and anti-androgens induce differential gene expression in prostate cancer.
MA5-12135 was used in immunohistochemistry to study the differential effects of chemical castration and anti-androgens on gene expression in prostate cancer
|Lehmusvaara S,Erkkilä T,Urbanucci A,Waltering K,Seppälä J,Larjo A,Tuominen VJ,Isola J,Kujala P,Lähdesmäki H,Kaipia A,Tammela TLj,Visakorpi T||The Journal of pathology (227:336)||2012|
A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma.
MA5-12135 was used in immunohistochemistry to study chordoid menigioma and the significance of the proportion of chordoid morphology for the risk of recurrence
|Lin JW,Lu CH,Lin WC,Wu YT,Huang YJ,Shih FY,Ho JT,Chuang MJ||Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia (19:836)||2012|
Identification of distinct gene expression profiles between esophageal squamous cell carcinoma and adjacent normal epithelial tissues.
MA5-12135 was used in immunohistochemistry to use cDNA arrays to study the distinct gene expression profiles of esophageal squamous cell carcinoma cells and the surrounding normal tissue
|Tao Y,Chai D,Ma L,Zhang T,Feng Z,Cheng Z,Wu S,Qin Y,Lai M||The Tohoku journal of experimental medicine (226:301)||2012|
The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution.
MA5-12135 was used in immunohistochemistry to study the prognostic value of MGMT and EGFR expression in primary gliosarcoma
|Lin JW,Wu YT,Chang IW||Indian journal of pathology and microbiology (54:683)||2012|
Routine dual-color immunostaining with a 3-antibody cocktail improves the detection of small cancers in prostate needle biopsies.
MA5-12135 was used in immunohistochemistry to evaluate a dual-color immunostaining protocol to detect small cancers in prostate needle biopsies
|Tolonen TT,Kujala PM,Laurila M,Tirkkonen M,Ilvesaro J,Tuominen VJ,Tammela TL,Isola J||Human pathology (42:1635)||2011|
Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution.
MA5-12135 was used in immunohistochemistry to study the clinicopathology of clear cell meningioma with frequent chordoid features and aggressive behavior
|Chen HK,Wu YT,Lin YJ,Lin JW||Journal of neuro-oncology (103:551)||2011|
Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution.
MA5-12135 was used in immunohistochemistry to study the clinicopathology of 11 cases of chordoid meningioma
|Lin JW,Ho JT,Lin YJ,Wu YT||Journal of neuro-oncology (100:465)||2010|
Differences in the structural features of atypical adenomatous hyperplasia and low-grade prostatic adenocarcinoma.
MA5-12135 was used in immunohistochemistry to investigate the diagnostic histological differences between atypical adenomatous hyperplasia and low-grade prostatic adenocarcinoma
|Midi A,Tecimer T,Bozkurt S,Ozkan N||Indian journal of urology : IJU : journal of the Urological Society of India (24:169)||2008|
Chronic inflammation in benign prostate hyperplasia is associated with focal upregulation of cyclooxygenase-2, Bcl-2, and cell proliferation in the glandular epithelium.
MA5-12135 was used in immunohistochemistry to study the upregulation of inflammatory pathways in benign prostatic hyperplasia and their possible role in prostate cancer pathogenesis
|Wang W,Bergh A,Damber JE||The Prostate (61:60)||2004|