|Tested species reactivity||Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human epidermal Keratin|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13150 targets Cytokeratin High Molecular Weight in IF and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples.
The MA5-13150 immunogen is human epidermal Keratin.
This antibody detects basic (Type II) cytokeratins: Cytokeratin 1, Cytokeratin 2, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 6, Cytokeratin 7 and Cytokeratin 8.
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Novel immunofluorescence protocol for multimarker assessment of putative disseminating breast cancer stem cells.
MA5-13150 was used in immunohistochemistry to investigate the efficiency of a new method for the assessment of putative disseminating breast cancer stem cells
|Balic M,Rapp N,Stanzer S,Lin H,Strutz J,Szkandera J,Daidone MG,Samonigg H,Cote RJ,Dandachi N||Applied immunohistochemistry and molecular morphology : AIMM (19:33)||2011|
Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.
MA5-13150 was used in immunohistochemistry to investigate tumourigenesis in non-mammary tissues in the MNU-induced mammary tumour model
|Esendagli G,Yilmaz G,Canpinar H,Gunel-Ozcan A,Guc MO,Guc D||Laboratory animals (43:60)||2009|
BMP4 signaling regulates formation of Hertwig's epithelial root sheath during tooth root development.
MA5-13150 was used in immunohistochemistry to study the role of BMP4 signaling in regulating the formation of Hertwig's epithelial root sheath during tooth root development
|Hosoya A,Kim JY,Cho SW,Jung HS||Cell and tissue research (333:503)||2008|
Graft-versus-host-like disease complicating thymoma: lack of AIRE expression as a cause of non-hereditary autoimmunity?
MA5-13150 was used in immunohistochemistry to report on three cases of graft-versus-host-like enterocolonopathy
|Offerhaus GJ,Schipper ME,Lazenby AJ,Montgomery E,Morsink FH,Bende RJ,Musler AR,van Lier RA,van Noesel CJ||Immunology letters (114:31)||2007|
Mediastinal intraoperative radioisotope sentinel lymph node mapping in non-small-cell lung cancer.
MA5-13150 was used in immunohistochemistry to develop a method to map sentinel lymph nodes in non-small cell lung cancer patients
|Atinkaya C,Ozlem Küçük N,Koparal H,Aras G,Sak SD,Ozdemir N||Nuclear medicine communications (26:717)||2005|
Cytokeratin expression in lichen amyloidosus and macular amyloidosis.
MA5-13150 was used in immunohistochemistry to investigate the expression of cytokeratins in lichen and macular amyloidosis and their diagnostic value
|Apaydin R,Gürbüz Y,Bayramgürler D,Müezzinoglu B,Bilen N||Journal of the European Academy of Dermatology and Venereology : JEADV (18:305)||2004|
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
MA5-13150 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
|Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS||Research in veterinary science (88:285)||2010|
An immunohistochemical study of feline endometrial adenocarcinoma.
MA5-13150 was used in immunohistochemistry - paraffin section to characterize feline endometrial adenocarcinomas immunohistochemically
|Gil da Costa RM,Santos M,Amorim I,Lopes C,Pereira PD,Faustino AM||Journal of comparative pathology (140:254)||2009|