Immunofluorescence analysis of Cytokeratin Low Molecular Weight was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8/18 Monoclonal Antibody (Product # MA5-13144) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Bovine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Rat, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human epidermal keratin|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/mL|
|Immunofluorescence (IF)||5 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13144 targets Cytokeratin Low Molecular Weight in WB, IF and IHC (P) applications and shows reactivity with Bovine, Chicken, Human, mouse, Non-human primate, Rabbit, and Rat samples. This antibody is not recommended for NIH-3T3 cells in immunofluorescent applications.
The MA5-13144 immunogen is human epidermal keratin.
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fumarate induces redox-dependent senescence by modifying glutathione metabolism.
MA5-13144 was used in immunohistochemistry - paraffin section to study how redox-dependent senescence is induced by fumarate to modify glutathione metabolism
|Zheng L,Cardaci S,Jerby L,MacKenzie ED,Sciacovelli M,Johnson TI,Gaude E,King A,Leach JD,Edrada-Ebel R,Hedley A,Morrice NA,Kalna G,Blyth K,Ruppin E,Frezza C,Gottlieb E||Nature communications (6:null)||2015|
An immunohistochemical study of feline endometrial adenocarcinoma.
MA5-13144 was used in immunohistochemistry - paraffin section to characterize feline endometrial adenocarcinomas immunohistochemically
|Gil da Costa RM,Santos M,Amorim I,Lopes C,Pereira PD,Faustino AM||Journal of comparative pathology (140:254)||2009|
Association of Merkel cell polyomavirus infection with EGFR mutation status in Chinese non-small cell lung cancer patients.
MA5-13144 was used in immunohistochemistry to study Chinese non-small cell lung cancer patients to deterine if there is an association between polyomavirus infection of Merkel cells and EGFR hotspot mutations
|Xu S,Jiang J,Yu X,Sheng D,Zhu T,Jin M||Lung cancer (Amsterdam, Netherlands) (83:341)||2014|
A clinicopathological study of the significance of the proportion of choroid morphology in chordoid meningioma.
MA5-13144 was used in immunohistochemistry to study chordoid menigioma and the significance of the proportion of chordoid morphology for the risk of recurrence
|Lin JW,Lu CH,Lin WC,Wu YT,Huang YJ,Shih FY,Ho JT,Chuang MJ||Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia (19:836)||2012|
The prognostic impact of O6-methylguanine DNA methyltransferase and epidermal growth factor receptor expressions on primary gliosarcoma: a clinicopathologic and immunohistochemical study of seven cases at a single institution.
MA5-13144 was used in immunohistochemistry to study the prognostic value of MGMT and EGFR expression in primary gliosarcoma
|Lin JW,Wu YT,Chang IW||Indian journal of pathology and microbiology (54:683)||2012|
Rhabdoid papillary meningioma: a clinicopathologic case series study.
MA5-13144 was used in immunohistochemistry to study the clinicopathology of a series of cases of rhabdoid papillary meningioma
|Wu YT,Ho JT,Lin YJ,Lin JW||Neuropathology : official journal of the Japanese Society of Neuropathology (31:599)||2011|
Clear cell meningioma with frequent chordoid features and aggressive behavior: a clinicopathologic study of ten cases at a single institution.
MA5-13144 was used in immunohistochemistry to study the clinicopathology of clear cell meningioma with frequent chordoid features and aggressive behavior
|Chen HK,Wu YT,Lin YJ,Lin JW||Journal of neuro-oncology (103:551)||2011|
Novel immunofluorescence protocol for multimarker assessment of putative disseminating breast cancer stem cells.
MA5-13144 was used in immunohistochemistry to investigate the efficiency of a new method for the assessment of putative disseminating breast cancer stem cells
|Balic M,Rapp N,Stanzer S,Lin H,Strutz J,Szkandera J,Daidone MG,Samonigg H,Cote RJ,Dandachi N||Applied immunohistochemistry and molecular morphology : AIMM (19:33)||2011|
Chordoid meningioma: a clinicopathologic study of 11 cases at a single institution.
MA5-13144 was used in immunohistochemistry to study the clinicopathology of 11 cases of chordoid meningioma
|Lin JW,Ho JT,Lin YJ,Wu YT||Journal of neuro-oncology (100:465)||2010|
Melanotic Xp11 translocation renal cancer: a case with PSF-TFE3 gene fusion and up-regulation of melanogenetic transcripts.
MA5-13144 was used in immunohistochemistry to report on a case of melanotic Xp11 translocation renal cancer
|Chang IW,Huang HY,Sung MT||The American journal of surgical pathology (33:1894)||2009|
Coexistence of different tissue tumourigenesis in an N-methyl-N-nitrosourea-induced mammary carcinoma model: a histopathological report in Sprague-Dawley rats.
MA5-13144 was used in immunohistochemistry to investigate tumourigenesis in non-mammary tissues in the MNU-induced mammary tumour model
|Esendagli G,Yilmaz G,Canpinar H,Gunel-Ozcan A,Guc MO,Guc D||Laboratory animals (43:60)||2009|
Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.
MA5-13144 was used in immunohistochemistry to study micrometastases and skip metastases in carcinoma of the colon and rectum
|Yagci G,Unlu A,Kurt B,Can MF,Kaymakcioglu N,Cetiner S,Tufan T,Sen D||International journal of colorectal disease (22:167)||2007|
Mediastinal intraoperative radioisotope sentinel lymph node mapping in non-small-cell lung cancer.
MA5-13144 was used in immunohistochemistry to develop a method to map sentinel lymph nodes in non-small cell lung cancer patients
|Atinkaya C,Ozlem Küçük N,Koparal H,Aras G,Sak SD,Ozdemir N||Nuclear medicine communications (26:717)||2005|
Ectopic thyroid in the gallbladder: report of a case.
MA5-13144 was used in immunohistochemistry to describe an unusual case of ectopic thyroid tissue
|Ihtiyar E,Isiksoy S,Algin C,Sahin A,Erkasap S,Yasar B||Surgery today (33:777)||2003|
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
MA5-13144 was used in western blot to study the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines
|Chang CY,Chiou PP,Chen WJ,Li YH,Yiu JC,Cheng YH,Chen SD,Lin CT,Lai YS||Research in veterinary science (88:285)||2010|
Possible association between HPV16 E7 protein level and cytokeratin 19.
MA5-13144 was used in western blot to study the association between HPV16 E7 protein levels and cytokeratin 19
|Favia G,Kanduc D,Lo Muzio L,Lucchese A,Serpico R||International journal of cancer (111:795)||2004|